Metastatic Small Cell Malignant Melanoma: A Case Requiring Immunoelectronmicroscopy for the Demonstration of Lattice-deficient Melanosomes

Autor:	Saumitra S Banerjee, Jill Moss, Brian P Eyden, Ian Shore
Rok vydání:	2005
Předmět:	Male Pathology medicine.medical_specialty Skin Neoplasms Biology Cell morphology Small-cell carcinoma Pathology and Forensic Medicine Diagnosis Differential Neoplasms Multiple Primary Cytokeratin Immunolabeling Microscopy Electron Transmission Structural Biology medicine Humans Microscopy Immunoelectron Melanoma Aged Melanosomes S100 Proteins Histology medicine.disease Immunohistochemistry Carcinoma Basal Cell Small-cell melanoma Lymphatic Metastasis Ultrastructure Keratins Neoplasm Recurrence Local
Zdroj:	Ultrastructural Pathology. 29:71-78
ISSN:	1521-0758 0191-3123
DOI:	10.1080/01913120590910612
Popis:	A case of metastatic malignant melanoma exhibiting small cell morphology is described. The patient had had a previous primary nodular small cell melanoma. The metastatic tumor was examined by conventional histology, light microscope immunohistochemistry, conventional electron microscopy, and ultrastructural immunolabeling. It consisted of small cells, which, however, varied in size and were present in distinct but merging areas. Tumor cells were negative for S-100 protein and very focally positive for cytokeratin: these findings in combination with small cell morphology suggested the possibility of small cell carcinoma. However, other melanocytic markers were positive. Neuroendocrine markers were negative. By electron microscopy, tumor cells lacked unambiguous melanosomes but contained paranuclear aggregates of nondescript granules. Following ultrastructural immunolabeling, these were found to be decorated with gold-labeled HMB-45 antibodies, thereby confirming them as lattice-deficient melanosomes. This tumor is an uncommon example of malignant melanoma where immunoultrastructural analysis helped clarify the nature of otherwise nondescript granules as true but lattice-deficient melanosomes. This is also the first case of small cell melanoma to be studied by electron microscopy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8866babb14a562384dda6f9fc1a427f4 https://doi.org/10.1080/01913120590910612 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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