Phototropins Mediate Chloroplast Movement in Phalaenopsis aphrodite (Moth Orchid)
| Autor: | Yi Jyun Lin, Yu Chung Chen, Wen Chi Chang, Swee-Suak Ko, Kuan Chieh Tseng |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Phototropins Phototropin Chloroplasts DNA Complementary Light Physiology Gene Expression Plant Science Protein Serine-Threonine Kinases 01 natural sciences Phalaenopsis aphrodite 03 medical and health sciences Arabidopsis Botany Gene Silencing Photosynthesis Orchidaceae Phototropism In Situ Hybridization Phylogeny Phalaenopsis equestris Plant Proteins biology Chloroplast avoidance movement Arabidopsis Proteins food and beverages Cell Biology General Medicine biology.organism_classification Plants Genetically Modified Chloroplast Plant Leaves 030104 developmental biology Mutation Phalaenopsis 010606 plant biology & botany |
| Zdroj: | Plantcell physiology. 60(10) |
| ISSN: | 1471-9053 |
| Popis: | Chloroplast movement is important for plants to avoid photodamage and to perform efficient photosynthesis. Phototropins are blue light receptors in plants that function in chloroplast movement, phototropism, stomatal opening, and they also affect plant growth and development. In this study, full-length cDNAs of two PHOTOTROPIN genes, PaPHOT1 and PaPHOT2, were cloned from a moth orchid Phalaenopsis aphrodite, and their functions in chloroplast movement were investigated. Phylogenetic analysis showed that PaPHOT1 and PaPHOT2 orthologs were highly similar to PHOT1 and PHOT2 of the close relative Phalaenopsis equestris, respectively, and clustered with monocots PHOT1 and PHOT2 orthologs, respectively. Phalaenopsis aphrodite expressed a moderate level of PaPHOT1 under low blue light of 5 μmol�m−2�s−1 (BL5) and a high levels of PaPHOT1 at >BL100. However, PaPHOT2 was expressed at low levels at BL100. Analysis of light-induced chloroplast movements using the SPAD method indicated that orchid accumulated chloroplasts at BL25 and significant chloroplast avoidance movement was observed at >BL100. Virus-induced gene silencing of PaPHOTs in orchids showed decreased gene expression of PaPHOTs and reduced both chloroplast accumulation and avoidance responses. Heterologous expression of PaPHOT1 in Arabidopsis phot1phot2 double mutant recovered chloroplast accumulation response at BL5, but neither PaPHOT1 nor PaPHOT2 was able to restore mutant chloroplast avoidance at BL100. Overall, this study showed that phototropins mediate chloroplast movement in Phalaenopsis orchid is blue light-dependent but their function is slightly different from Arabidopsis which might be due to gene evolution. |
| Databáze: | OpenAIRE |
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