Overexpression of MHC class I in muscle of lymphocyte-deficient mice causes a severe myopathy with induction of the unfolded protein response
| Autor: | François-Jérôme Authier, Olivier Boyer, Laurent Drouot, Manuel Fréret, Claire Dauly, Pascal Cosette, Antoine Obry, Sandra Ahmed-Lacheheb, Sahil Adriouch |
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| Rok vydání: | 2012 |
| Předmět: |
T-Lymphocytes
Muscle Fibers Skeletal Intracellular Space Muscle Proteins chemical and pharmacologic phenomena Mice Transgenic Human leukocyte antigen Mice SCID Major histocompatibility complex Pathology and Forensic Medicine Myositis Inclusion Body Mice Immune system Muscular Diseases MHC class I medicine Animals Humans Myopathy biology Muscles Histocompatibility Antigens Class I Acquired immune system medicine.disease Endoplasmic Reticulum Stress Molecular biology Immunohistochemistry Cell biology Up-Regulation biology.protein Unfolded Protein Response Inclusion body myositis medicine.symptom CD8 |
| Zdroj: | The American journal of pathology. 183(3) |
| ISSN: | 1525-2191 |
| Popis: | Muscle fibers do not normally express major histocompatibility complex class I (MHC-I) molecules, and their reexpression is a hallmark of inflammatory myopathies. It has been shown in mice that overexpression of MHC-I induces a poorly inflammatory myositis accompanied by the unfolded protein response (UPR), but it is unclear whether it is attributable to T-cell-mediated MHC-I-dependent immune responses or to MHC-I forced expression per se. Indeed, besides presenting antigenic peptides to CD8(+) T cells, MHC-I may also possibly exert nonimmunologic, yet poorly understood pathogenic effects. Thus, we investigated the pathogenicity of MHC-I expression in muscle independently of its immune functions. HT transgenic mice that conditionally overexpress H-2K(b) in muscle were bred to an immunodeficient Rag2(-/-) background. The muscle proteome was analyzed by label-free high-resolution protein quantitation and Western blot. Despite the absence of adaptive immunity, HT Rag2(-/-) mice developed a very severe myopathy associated with the cytoplasmic accumulation of H-2K(b) molecules. The UPR was manifest by up-regulation of characteristic proteins. In humans, we found that HLA class I molecules not only were expressed at the sarcolemma but also could accumulate intracellularly in some patients with inclusion body myositis. Accordingly, the UPR was triggered as a function of the degree of HLA accumulation in myofibers. Hence, reexpression of MHC-I in normally negative myofibers exerts pathogenic effects independently of its immune function. |
| Databáze: | OpenAIRE |
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