Prevalence of chronic rhinosinusitis in Sao Paulo
| Autor: | Francini Grecco de Melo Padua, Richard Louis Voegels, Ricardo Ferreira Bento, Fábio de Rezende Pinna, Thiago Freire Pinto Bezerra, Claus Bachert, Renata Ribeiro de Mendonça Pilan, Renata Lopes Mori, Claudina Perez-Novo |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Urban Population Drug resistance Pharmacology Pathogenesis Young Adult Glucocorticoid receptor Prevalence medicine Cluster Analysis Humans Nasal polyps Sinusitis Child Aged Rhinitis Aged 80 and over business.industry Ligand binding assay Smoking General Medicine Middle Aged medicine.disease Ligand (biochemistry) Blot Cytosol Otorhinolaryngology Chronic Disease Female business Brazil |
| Zdroj: | Rhinology journal. 50:129-138 |
| ISSN: | 0300-0729 |
| DOI: | 10.4193/rhino11.256 |
| Popis: | BACKGROUND: Glucocorticoids (GCs) are considered drugs of choice for treating nasal polyps (NPs). However, a subset of patients shows a limited clinical response even to high doses of GCs. Altered expression of glucocorticoid receptors (GRs), namely GR-alpha; and GR-beta;, is a potential mechanism underlying GC insensitivity. GCs modulate the expression of several cytokines, including transforming growth factor-beta (TGF-beta), which may contribute to cellular proliferation in NPs. The study investigates some biomolecular features of GC-resistant NPs, and examines possible differences from normal mucosa (NM). METHODOLOGY: Radioligand binding assay (binding) was used to determine GR-alpha; binding capacity; Western blotting was used to evaluate GR-alpha;, GR-beta;, and TGF-beta; expression and GR-alpha; subcellular distribution. NPs were sampled in 32 patients during ethmoidectomy; NM was taken from 15 healthy patients during rhinoplasty. RESULTS: GR-alpha; was present in NPs and NM, with lower affinity for the ligand in NPs. GR-alpha; was prevalent in the cytosol of NPs that were GR-alpha-negative to the binding assay. GR-beta was expressed in NPs and absent in the majority of NM. TGF-beta1 expression was higher in NPs than in NM. CONCLUSIONS: GR-beta and TGF-beta1 might be involved in NP pathogenesis, but their role in modulating GC sensitivity is still unclear. |
| Databáze: | OpenAIRE |
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