Tailored Mobility in a Zeolite Imidazolate Framework (ZIF) Antibody Conjugate*
| Autor: | Sebastian Beyer, Franziska Emmerling, Carlos Abad, Carsten Prinz, Ander Chapartegui-Arias, Teodor Tchipilov, Rudolf J. Schneider, Anna Raysyan, Carsten Jaeger, Ana M. Belenguer |
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| Přispěvatelé: | Chapartegui-Arias, Ander [0000-0002-0889-7356], Raysyan, Anna [0000-0002-2760-327X], Belenguer, Ana M [0000-0002-0443-4856], Jaeger, Carsten [0000-0003-2192-1792], Tchipilov, Teodor [0000-0003-0755-8595], Prinz, Carsten [0000-0001-8586-5430], Abad, Carlos [0000-0003-3928-3258], Beyer, Sebastian [0000-0001-7945-1477], Schneider, Rudolf J [0000-0003-2228-1248], Emmerling, Franziska [0000-0001-8528-0301], Apollo - University of Cambridge Repository |
| Rok vydání: | 2021 |
| Předmět: |
Hot Paper
010402 general chemistry 01 natural sciences Horseradish peroxidase Catalysis chemistry.chemical_compound Tandem Mass Spectrometry Imidazolate medicine antibodies metal-organic frameworks Horseradish Peroxidase Full Paper medicine.diagnostic_test biology 010405 organic chemistry Organic Chemistry General Chemistry Full Papers Combinatorial chemistry Fluorescence 0104 chemical sciences immunoassays zeolite analogues chemistry Covalent bond Immunoassay biology.protein Zeolites Metal-organic framework nanoparticles Conjugate Zeolitic imidazolate framework Chromatography Liquid |
| Zdroj: | Chemistry (Weinheim an Der Bergstrasse, Germany) |
| DOI: | 10.17863/cam.70266 |
| Popis: | Zeolitic imidazolate framework (ZIF) hybrid fluorescent nanoparticles and ZIF antibody conjugates have been synthesized, characterized, and employed in lateral‐flow immunoassay (LFIA). The bright fluorescence of the conjugates and the possibility to tailor their mobility gives a huge potential for diagnostic assays. An enzyme‐linked immunosorbent assay (ELISA) with horseradish peroxidase (HRP) as label, proved the integrity, stability, and dispersibility of the antibody conjugates, LC‐MS/MS provided evidence that a covalent link was established between these metal‐organic frameworks and lysine residues in IgG antibodies. Framing an assay: We present nanosized zeolitic imidazolate frameworks (ZIFs) conjugated to immunoglobulin G (IgG) antibodies by using a strong and flexible covalent amino‐alkyl link. These nanoZIF‐IgG particles display good mobility and an excellent behaviour in dispersion. Their application to antibody‐based bioanalytical assays, including enzyme‐linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA), was examined. |
| Databáze: | OpenAIRE |
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