High production of L-glutamic acid from date juice extracts by Corynebacterium glutamicum using fed-batch cultures: pulsed and continuous feeding modes
| Autor: | Mouffok Abdenacer, Nancib Aïcha, Boudrant Joseph, Nancib Nabil |
|---|---|
| Přispěvatelé: | Laboratoire Réactions et Génie des Procédés (LRGP), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Nature and Life Sciences, Ferhat Abbas University-Setif 1, 19000 Setif |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Pulmonary and Respiratory Medicine Geography (General) 0303 health sciences feeding mode Chemistry Science date juice Continuous feeding Glutamic acid 01 natural sciences Corynebacterium glutamicum [SPI]Engineering Sciences [physics] 03 medical and health sciences 010608 biotechnology G1-922 Feeding mode l-glutamic acid fed-batch fermentation Pediatrics Perinatology and Child Health Food science corynebacterium glutamicum ComputingMilieux_MISCELLANEOUS 030304 developmental biology |
| Zdroj: | Acta Scientifica Naturalis, Vol 8, Iss 1, Pp 37-50 (2021) Acta Scientifica Naturalis Acta Scientifica Naturalis, K. Preslavsky University, 2021, ⟨10.2478/asn-2021-0004⟩ |
| ISSN: | 2367-5144 |
| Popis: | In the present work, L-glutamic acid production by Corynebacterium glutamicum fermentation on date juice extracts applying two fed-batch feeding modes, pulsed and continuous, were investigated. According to the obtained results, the continuous feeding fed-batch mode was found to be the most efficient process. Moreover the continuous feeding rate mode with a feeding medium containing date juice sugars enriched with ammonium sulfate was found even more favorable as it enhances the L-glutamic acid production by approximately 2.35 fold more than the batch culture and by about 1.17 fold more than the pulsed feeding. In this respect, comparing the traditional batch culture to the continuously fed culture with a medium containing date juice sugars with ammonium sulfate showed increases of 135.47% in L-glutamic acid production, 104% in productivity, 39.09% in biomass, and 47.69% in the yield respectively allowing us to reach a final L-glutamic acid concentration of about 138 g/L, the highest ever published. |
| Databáze: | OpenAIRE |
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