Serum metabolomics reveals the mechanistic role of functional foods and exercise for obesity management in rats
| Autor: | Naglaa M. Ammar, Nora M. El-Sheikh, Nadia S. Metwally, Abdel Nasser El Gendy, Mohamed A. Farag, Abdel Hamid Z. Abdel Hamid, Tahani E. Kholeif |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Drug media_common.quotation_subject Metabolite Clinical Biochemistry Pharmaceutical Science Physiology 030204 cardiovascular system & hematology Diet High-Fat Gas Chromatography-Mass Spectrometry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food Metabolomics Functional Food Drug Discovery medicine Animals Obesity Spectroscopy media_common chemistry.chemical_classification Red cabbage Chemistry Aminobutyrates medicine.disease food.food Rats Amino acid Phenylethanolamine 030104 developmental biology Biochemistry Gas chromatography–mass spectrometry |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 142:91-101 |
| ISSN: | 0731-7085 |
| Popis: | Obesity is one of the independent risk factors for several health problems, leading to metabolic perturbations and for which analytical approaches i.e., "metabolomics" is needed to monitor the underlying metabolic changes. In this study, obesity associated changes were assessed via serum metabolites analysis of obese rats fed on high fat diet. Obese rats were subsequently treated with different functional foods used for obesity management including pomegranate, grapefruit, and red cabbage in parallel to swimming exercise. Serum samples were analyzed using gas chromatography-mass spectrometry (GC-MS) followed by multivariate data analysis to classify samples and determine if such treatments can help revert obesity related metabolic changes back to normal status. Results led to the identification of several novel metabolites biomarkers for obesity related to lipids, amino acids and central tricarboxylic acid (TCA) pathways. Distinct variations in metabolite levels were recorded in obese rats compared to normal ones including l-aspartic, l-alanine, l-glutamine, l-glycine, phenylethanolamine, α-aminobutyric acid and β-hydroxybutyric acid. Metabolomics approach developed herein provides novel insight onto the metabolic disturbances associated with obesity, which will assist in future drug design that can help mitigate against such changes. |
| Databáze: | OpenAIRE |
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