Lack of GLYCOLATE OXIDASE1, but Not GLYCOLATE OXIDASE2, Attenuates the Photorespiratory Phenotype of CATALASE2-Deficient Arabidopsis
| Autor: | Alexey Shapiguzov, Alisdair R. Fernie, Saleh Alseekh, Frank Van Breusegem, Cezary Waszczak, Yves Van de Peer, Patrick Willems, Pavel Kerchev, Per Mühlenbock, Jingjing Huang, Riet De Smet, Zhen Li, Aleksandra Lewandowska, Jaakko Kangasjärvi, Joris Messens, Katrien Van Der Kelen, Frank A. Hoeberichts |
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| Přispěvatelé: | Biosciences, Plant Biology, Plant ROS-Signalling, Viikki Plant Science Centre (ViPS), Structural Biology Brussels, Faculty of Sciences and Bioengineering Sciences, Department of Bio-engineering Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Light Physiology Research Articles - Focus Issue Cell Respiration Mutant Arabidopsis Plant Science ASCORBATE PEROXIDASE METABOLISM 01 natural sciences Evolution Molecular 03 medical and health sciences Genetics Arabidopsis thaliana OXYGEN GENE NETWORK PROTECTION Photosynthesis OXIDATIVE STRESS PLANT MAXIMUM-LIKELIHOOD 1183 Plant biology microbiology virology CLIMATE-CHANGE biology Arabidopsis Proteins Biology and Life Sciences Plant physiology Peroxisome biology.organism_classification Phenotype Glycolates S-NITROSYLATED PROTEINS Alcohol Oxidoreductases 030104 developmental biology Biochemistry CELL-DEATH Mutation Metabolome Photorespiration Oxidation-Reduction Flux (metabolism) 010606 plant biology & botany |
| Zdroj: | PLANT PHYSIOLOGY |
| ISSN: | 0032-0889 1532-2548 |
| Popis: | The genes coding for the core metabolic enzymes of the photorespiratory pathway that allows plants with C3-type photosynthesis to survive in an oxygen-rich atmosphere, have been largely discovered in genetic screens aimed to isolate mutants that are unviable under ambient air. As an exception, glycolate oxidase (GOX) mutants with a photorespiratory phenotype have not been described yet in C3 species. Using Arabidopsis (Arabidopsis thaliana) mutants lacking the peroxisomal CATALASE2 (cat2-2) that display stunted growth and cell death lesions under ambient air, we isolated a second-site loss-of-function mutation in GLYCOLATE OXIDASE1 (GOX1) that attenuated the photorespiratory phenotype of cat2-2. Interestingly, knocking out the nearly identical GOX2 in the cat2-2 background did not affect the photorespiratory phenotype, indicating that GOX1 and GOX2 play distinct metabolic roles. We further investigated their individual functions in single gox1-1 and gox2-1 mutants and revealed that their phenotypes can be modulated by environmental conditions that increase the metabolic flux through the photorespiratory pathway. High light negatively affected the photosynthetic performance and growth of both gox1-1 and gox2-1 mutants, but the negative consequences of severe photorespiration were more pronounced in the absence of GOX1, which was accompanied with lesser ability to process glycolate. Taken together, our results point toward divergent functions of the two photorespiratory GOX isoforms in Arabidopsis and contribute to a better understanding of the photorespiratory pathway. |
| Databáze: | OpenAIRE |
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