Coupled biocatalysts applied to the synthesis of nucleosides
| Autor: | Adolfo M. Iribarren, Marisa Lia Taverna Porro, Elizabeth Sandra Lewkowicz, Javier Marcelo Montserrat, Rosario Médici |
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| Předmět: |
Purine
Pyrimidine Guanine Adenosine Deaminase Synthesis of nucleosides Chemical synthesis Catalysis Ciencias Biológicas chemistry.chemical_compound Organic chemistry Pentosyltransferases MODIFIED NUCLEOSIDES Arthrobacter chemistry.chemical_classification ENZYMATIC SYNTHESIS Phosphotransferases Ciencias Químicas Nucleosides General Medicine Bioquímica y Biología Molecular Furanose Phosphopentomutase ENZYME IMMOBILIZATION Química Orgánica chemistry Sugar Phosphates Nucleoside CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Scopus-Elsevier |
| Popis: | Biocatalytic procedures offer a good alternative to the chemical synthesis of nucleosides since biocatalyzed reactions are regio- and stereoselective and afford reduced by-products contents. Among them, enzymatic transglycosylation between a pyrimidine nucleoside and a purine base catalyzed by nucleoside phosphorylases or microorganisms that contain them, has attracted considerable attention. In addition, the combination to other enzymatic steps has been explored. In this work we investigate the coupled action of nucleoside phosphorylases with other enzymatic activities: deaminase and phosphopentomutase. Unlike the preparation of other purine nucleosides, transglycosylation from a pyrimidine nucleoside and guanine is difficult because of the low solubility of this base. Therefore, another strategy, based on microbial transglycosylation followed by deamination, is here explored. The direct use of furanose 1-phosphate, the intermediate in the transglycosylation reaction, is an attractive alternative when pyrimidine nucleosides are not available. Its preparation from the more stable furanose 5-phosphate and phosphopentomutase is here applied to different sugars and bases. Fil: Médici, Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Área Química. Laboratorio de Biotransformaciones; Argentina Fil: Taverna Porro, Marisa Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Lewkowicz, Elizabeth Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Área Química. Laboratorio de Biotransformaciones; Argentina Fil: Montserrat, Javier Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de General Sarmiento; Argentina Fil: Iribarren, Adolfo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de Quilmes; Argentina |
| Databáze: | OpenAIRE |
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