Milk Kefir therapy reduces inflammation and alveolar bone loss on periodontitis in rats
Autor: | Paula Goes, Luane Macêdo de Sousa, Fabio Miyajima, Delane Viana Gondim, Jonas Nogueira Ferreira Maciel Gusmão, Thays Allane Cordeiro Maia, Lorena Vasconcelos Vieira, Karuza Maria Alves Pereira |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Male Periodontium medicine.medical_specialty Periodontal Ligament Alveolar Bone Loss Inflammation Bone resorption RM1-950 law.invention 03 medical and health sciences Probiotic 0302 clinical medicine Kefir law Internal medicine medicine Periodontal fiber Animals Rats Wistar Periodontitis Dental alveolus Pharmacology business.industry Probiotics General Medicine X-Ray Microtomography medicine.disease Rats 030104 developmental biology Endocrinology 030220 oncology & carcinogenesis Fermentation Immunohistochemistry Cytokines Therapeutics. Pharmacology medicine.symptom Periodontal disease business |
Zdroj: | Biomedicine & Pharmacotherapy, Vol 139, Iss, Pp 111677-(2021) |
ISSN: | 0753-3322 |
Popis: | Periodontitis is a chronic inflammatory disease that affects the tooth-supporting tissues. This study evaluated the anti-inflammatory and antiresorptive effects of milk kefir (MK) on periodontitis in rats. Micro-Raman spectroscopy was performed on MK at different fermentation times to verify the presence of Lactobacillus kefiri. From these results, Wistar rats were divided into the following groups: C (Control); EP (experimental periodontitis); K1 (animals that received MK with one day of fermentation); K1+EP; K4 (animals without EP using MK with four days of fermentation) and K4+EP. MK was administered 28 days before EP induction and during the disease development period (11 days). On day 28, in the EP groups, periodontitis was induced. The animals were euthanized on day 39. The hemimaxillae were removed and the following parameters were evaluated: micro-Raman analysis of the presence of inflammation; histomorphometric analysis to quantify alveolar bone loss and immunohistochemistry for IL-6, TNF-α, IL-Iβ and IL-10 in the periodontal ligament. Micro-Raman analysis showed that four days fermentation MK has a higher intensity spectrum of L. kefiri. Furthermore, the administration of this probiotic reduced the intensity of the inflammation spectrum when compared to one day fermentation MK. It was observed that the animals from the K4+EP group showed significant reduction of alveolar bone loss, as well as a lower IL-6, TNF-α and IL-Iβ immunoexpression and a higher IL-10 immunoexpression, when compared to EP groups. We conclude that MK has anti-inflammatory and antiresorptive effects on periodontitis in rats and that these effects are fermentation time dependent. |
Databáze: | OpenAIRE |
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