Rostral ventromedial medulla connections in Cavia porcellus and their relation with tonic immobility defensive behavior: a biotinylated dextran amine neurotracing study
| Autor: | Leda Menescal-de-Oliveira, Luis Felipe Souza da Silva, Norberto Cysne Coimbra |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Neuroscience(all) Periaqueductal gray matter Guinea Pigs Biotin Cavia Horseradish peroxidase medicine Animals Periaqueductal Gray Microinjection Biotinylated dextran amine Medulla Fluorescent Dyes Medulla Oblongata biology Chemistry General Neuroscience Dextrans Immobility Response Tonic Anatomy Spinal cord biology.organism_classification RVM-spinal cord pathways medicine.anatomical_structure Nociception Cuneiform nucleus Spinal Cord nervous system biology.protein MEDULA ESPINHAL Rostral ventromedial medulla |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | The rostroventromedial medulla (RVM), together with the periaqueductal gray matter (PAG), constitutes the descendent antinociceptive system. Additionally, these structures mutually regulate defensive behaviors, including tonic immobility (TI) in guinea pigs. The current study was undertaken to evaluate the connections of the RVM with the PAG and the spinal cord in guinea pigs in order to provide an anatomical basis for the role played by RVM in the modulation of TI. To address this goal, five guinea pigs were treated with non-fluorescent biotinylated dextran amine (BDA) neurotracer by injection into the RVM. After four days of survival, the encephalon and spinal cord were removed from each rodent, and BDA labeling was visualized with a standard avidin-biotinylated horseradish peroxidase method through reaction with nickel-intensified peroxidase 3,3′-diaminobenzidine dihydrochloride. The microinjection of BDA into the RVM stained fibers in the ventral horn, dorsal horn and intermediate gray matter of the spinal cord. BDA-labeled fibers, terminal buttons suggesting synaptic contacts, and perikarya were found in the dorsomedial, dorsolateral, lateral and ventrolateral PAG, and neuronal somata were identified in the cuneiform nucleus. Together, the current data demonstrate neuroanatomical evidence that supports the role of the RVM in the modulation of TI defensive behavior. |
| Databáze: | OpenAIRE |
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