Analysis of Genetic Diversity and Development of SCAR Markers in a Mycogone perniciosa Population
| Autor: | Zhi-Qiang Wen, Cheng-Huan Li, Bingzhi Chen, Shuang Wang, Wei Wang, Xiao Li |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Genetic Markers
0106 biological sciences 0301 basic medicine Agaricus Population Fungus 01 natural sciences Applied Microbiology and Biotechnology Microbiology DNA sequencing 03 medical and health sciences Polymorphism (computer science) Vegetables Genetic variation DNA Fungal education Plant Diseases Genetics Genetic diversity education.field_of_study biology Genetic Variation General Medicine biology.organism_classification Random Amplified Polymorphic DNA Technique RAPD 030104 developmental biology Genetic marker Hypocreales Microsatellite Repeats 010606 plant biology & botany |
| Zdroj: | Current Microbiology. 73:9-14 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-016-1020-1 |
| Popis: | The fungus Mycogone perniciosa is a major pathogen of the common button mushroom Agaricus bisporus. Analysis of genetic diversity in M. Perniciosa may assist in developing methods for prophylaxis and treatment of M. Perniciosa infections. For this, it is necessary to classify M. Perniciosa into relevant class groups quickly and efficiently. Random amplified polymorphic DNA (RAPD), inter-simple sequence repeats (ISSR), and sequence-related amplified polymorphism (SRAP) markers were used to obtain genetic fingerprints and assess the genetic variation among 49 strains of M. perniciosa collected from different areas of Fujian Province in China. Analysis of DNA sequence polymorphism revealed two major distinct groups (Group I and Group II). Specific DNA fragments that were identified through RAPD, ISSR, and SRAP markers were sequenced and used for the designing of stable sequence-characterized amplified region (SCAR) markers. The resulting SCAR markers were then validated against the classified groups of M. perniciosa. |
| Databáze: | OpenAIRE |
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