Alnus Sibirica Extracts Suppress the Expression of Inflammatory Cytokines Induced by Lipopolysaccharides, Tumor Necrosis Factor-α, and Interferon-γ in Human Dermal Fibroblasts

Autor: Yelim Seo, Hye Soo Wang, Donghee Lee, Young-Won Kim, Hyoweon Bang, Young Wook Choi, Jae-Hong Ko, Seongtae Kim, Min Won Lee, Jeongyoon Choi, Hyemi Bae, Sunghee Moon, Inja Lim
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Molecules, Vol 24, Iss 16, p 2883 (2019)
Molecules
Volume 24
Issue 16
ISSN: 1420-3049
Popis: The effects of Alnus sibirica (AS) extracts on cytokine expression induced by inflammatory stimulants were examined in human dermal fibroblasts (HDFs) and RAW264.7 cells. The anti-oxidative effect and effect on cell viability of AS extracts were evaluated, and four extracts with the highest anti-oxidative effects were selected. HDFs and RAW264.7 cells were treated with inflammatory stimulants, and the expression of cytokines involved in acute (IL-6 and IL-10) and chronic (IL-18) inflammation, the initiation of the immune response (IL-33), and non-specific immune responses (IL-1&beta
IL-8, and TNF-&alpha
) were determined using a reverse-transcription polymerase chain reaction. LPS increased the expression of all the cytokines, except for IL-18
however, AS extracts, particularly AS2 and AS4, reduced this increase, and TNF-&alpha
treatment markedly increased the expression of cytokines related to non-specific immune responses. IFN-&gamma
treatment induced no significant changes, except for increased IL-33 expression in HDFs. AS extracts inhibited the increase in the expression of IL-33 and other cytokines in HDFs. Thus, the exposure of HDFs and RAW264.7 cells to inflammatory stimulants increased the expression of cytokines related to all the inflammatory processes. HDFs are involved not only in simple tissue regeneration but also in inflammatory reactions in the skin. AS2 and AS4 may offer effective therapy for related conditions.
Databáze: OpenAIRE
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