| Autor: |
Jun Yang, Peiling Dai, Meng Li, Man Tang, Qi Wu, Shujuan Liu, Qiang Zhao, Kenneth Yin Zhang |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Dalton transactions (Cambridge, England : 2003). 51(15) |
| ISSN: |
1477-9234 |
| Popis: |
Fluorescent/phosphorescent dual-emissive polymers or hybrids consisting of both fluorophore and phosphor have been used as self-calibrating probes and imaging reagents for cellular molecular oxygen. Oxygen selectively quenches the phosphorescence and the fluorescence serves as an internal reference. The phosphorescence/fluorescence ratio is used as a quantitative indicator of oxygen content. In wavelength-ratiometric probes, the fluorophore and phosphor are designed to emit at different wavelengths. It is easy to achieve spectral separation, but the phosphorescence/fluorescence ratio fluctuates due to the difference in the absorption and scattering of light at different wavelengths by biological samples. Herein we reported a lifetime-ratiometric luminescent polymeric probe where the fluorophore and phosphor emitted at the same wavelength. Spectral separation was achieved based on the difference in their excited-state lifetimes |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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