A Comparison Between Two Assays for Measuring Seminal Oxidative Stress and their Relationship with Sperm DNA Fragmentation and Semen Parameters
| Autor: | Andrew Dawkins, Jingyi Xie, Jesse Stone, Aideen P Killeen, Farley Gould, Sheryl T. Homa, Jonathan Ramsay, Anna M Vassiliou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male endocrine system lcsh:QH426-470 Semen DNA fragmentation Semen analysis medicine.disease_cause Article male infertility Male infertility Andrology 03 medical and health sciences 0302 clinical medicine Genetics medicine Humans oxidative stress DNA oxidation Genetics (clinical) Sperm motility Infertility Male reactive oxygen species 030219 obstetrics & reproductive medicine medicine.diagnostic_test Sperm Count Chemistry urogenital system medicine.disease Sperm Spermatozoa spermatogenesis Semen Analysis lcsh:Genetics 030104 developmental biology Sperm Motility chromatin Spermatogenesis Oxidative stress |
| Zdroj: | Genes Volume 10 Issue 3 Genes, Vol 10, Iss 3, p 236 (2019) |
| ISSN: | 2073-4425 |
| DOI: | 10.3390/genes10030236 |
| Popis: | Oxidative stress (OS) is a significant cause of DNA fragmentation and is associated with poor embryo development and recurrent miscarriage. The aim of this study was to compare two different methods for assessing seminal OS and their ability to predict sperm DNA fragmentation and abnormal semen parameters. Semen samples were collected from 520 men attending for routine diagnostic testing following informed consent. Oxidative stress was assessed using either a chemiluminescence assay to measure reactive oxygen species (ROS) or an electrochemical assay to measure oxidation reduction potential (sORP). Sperm DNA fragmentation (DFI) and sperm with immature chromatin (HDS) were assessed using sperm chromatin structure assay (SCSA). Semen analysis was performed according to WHO 2010 guidelines. Reactive oxygen species sORP and DFI are negatively correlated with sperm motility (p = 0.0012, 0.0002, < 0.0001 respectively) and vitality (p < 0.0001, 0.019, < 0.0001 respectively). The correlation was stronger for sORP than ROS. Reactive oxygen species (p < 0.0001), sORP (p < 0.0001), DFI (p < 0.0089) and HDS (p < 0.0001) were significantly elevated in samples with abnormal semen parameters, compared to those with normal parameters. Samples with polymorphonuclear leukocytes (PMN) have excessive ROS levels compared to those without (p < 0.0001), but sORP and DFI in this group are not significantly increased. DNA fragmentation was significantly elevated in samples with OS measured by ROS (p = 0.0052) or sORP (p = 0.004). The results demonstrate the multi-dimensional nature of oxidative stress and that neither assay can be used alone in the diagnosis of OS, especially in cases of leukocytospermia. |
| Databáze: | OpenAIRE |
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