An improved method for the isolation, quantitation, and identification of bile acids in rat feces
| Autor: | Gerald Salen, E. H. Mosbach, R. F. Raicht, Bertram I. Cohen |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Chromatography
Gas medicine.drug_class Biophysics Fraction (chemistry) Improved method Biology Chenodeoxycholic Acid Biochemistry Mass Spectrometry Bile Acids and Salts Feces Methods medicine Animals Sterol metabolism Molecular Biology Chromatography Bile acid Extraction (chemistry) Cholic Acids Cell Biology Isolation (microbiology) Rats Evaluation Studies as Topic Lithocholic Acid lipids (amino acids peptides and proteins) Chromatography Thin Layer Deoxycholic Acid |
| Zdroj: | Analytical Biochemistry. 64:567-577 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(75)90469-8 |
| Popis: | An improved method for the isolation and quantitation of bile acids from rat feces was developed. This method employs an initial Soxhlet extraction of the solid fecal material, esterification of the bile acid fraction with dry methanol/HCl and quantitation using a combination of tlc and glc techniques. In addition, identification of the individual components of the fecal bile acid fraction is accomplished by tlc and glc-ms. This method has proven useful for the quantitation and identification of the fecal bile acids during sterol metabolism measurements. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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