Two distinct motifs for Zic-r.a drive specific gene expression in two cell lineages
| Autor: | Izumi Oda-Ishii, Yutaka Satou, Deli Yu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Ascidians
animal structures Embryo Nonmammalian TBX6 Cell Gene Expression Biology 03 medical and health sciences 0302 clinical medicine Gene expression medicine Animals Cell Lineage Urochordata Molecular Biology Transcription factor Gene 030304 developmental biology Genetics 0303 health sciences Binding Sites Gene Expression Regulation Developmental Non-canonical-motif sites Embryo Zinc Fingers Tbx6 Zic In vitro Ciona intestinalis medicine.anatomical_structure Motif (music) T-Box Domain Proteins 030217 neurology & neurosurgery Developmental Biology Transcription Factors |
| Zdroj: | Development (Cambridge, England). 148(11) |
| ISSN: | 1477-9129 |
| Popis: | Zic-r.a, a maternal transcription factor, specifies posterior fate in ascidian embryos. However, its direct target, Tbx6-r.b, does not contain typical Zic-r.a-binding sites in its regulatory region. Using an in vitro selection assay, we found that Zic-r.a binds to sites dissimilar to the canonical motif, by which it activates Tbx6-r.b in a sub-lineage of muscle cells. These sites with non-canonical motifs have weak affinity for Zic-r.a; therefore, it activates Tbx6-r.b only in cells expressing Zic-r.a abundantly. Meanwhile, we found that Zic-r.a expressed zygotically in late embryos activates neural genes through canonical sites. Because different zinc-finger domains of Zic-r.a are important for driving reporters with canonical and non-canonical sites, it is likely that the non-canonical motif is not a divergent version of the canonical motif. In other words, our data indicate that the non-canonical motif represents a motif distinct from the canonical motif. Thus, Zic-r.a recognizes two distinct motifs to activate two sets of genes at two timepoints in development. This article has an associated ‘The people behind the papers’ interview. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|