Cloning, expression and function of phosphate transporter encoded gene in Oryza sativa L
| Autor: | Feng Ming, Bin Guo, Daleng Shen, Qun Lu, Wei Wang, Shanshan Zhang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Regulation of gene expression
Oryza sativa Transcription Genetic biology Gene Expression Profiling Molecular Sequence Data food and beverages Oryza Transporter biology.organism_classification Phosphate Molecular biology General Biochemistry Genetics and Molecular Biology Pichia pastoris Gene expression profiling chemistry.chemical_compound chemistry Gene Expression Regulation Plant Phosphate Transport Proteins Cloning Molecular General Agricultural and Biological Sciences Homologous recombination Gene Phylogeny General Environmental Science |
| Zdroj: | Science in China Series C: Life Sciences. 49:409-413 |
| ISSN: | 1862-2798 1006-9305 |
| DOI: | 10.1007/s11427-006-2030-1 |
| Popis: | OsPT6:1, a phosphate transporter encoding gene from the leaf samples of Oryza sativa, was identified through PCR with specifically designed primers. The phylogenetic analysis and the conserved amino acid residue site detection suggested OsPT6:1 a possible high-affinity phosphate transporter encoding gene. In situ hybridization and RT-PCR demonstrated the expression of OsPT6:1 in both roots and leaves. The peak expression signal was observed in mesophyll cells under low phosphorus (P) induction. A homologous recombination study indicated that OsPT6:1 can enhance the Pi uptake efficiency of Pichia pastoris. At the meantime, the introduction of OsPT6:1 was able to complement the Pi uptake function of yeast cells with high-affinity phosphate transporters deficient. Those results substantiated our contention that OsPT6:1 encoded a high-affinity phosphate transporter of Oryza sativa. |
| Databáze: | OpenAIRE |
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