Comparative methylation of ERVWE1/syncytin-1 and other human endogenous retrovirus LTRs in placenta tissues
| Autor: | Karine Ruel, Pascale Gerbaud, Jean-Louis Frendo, François Mallet, Juliette Gimenez, Vassilis Tsatsaris, Danièle Evain-Brion, Jean-Philippe Pichon, Cécile Montgiraud, Guy Oriol |
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| Rok vydání: | 2009 |
| Předmět: |
placenta
viruses LTR Molecular Sequence Data Endogenous retrovirus Biology Pregnancy Proteins syncytins Cell Line Tumor Genetics Humans Epigenetics Promoter Regions Genetic Molecular Biology RNA-Directed DNA Methylation Cells Cultured Regulation of gene expression Base Sequence Endogenous Retroviruses Terminal Repeat Sequences Gene Products env General Medicine Methylation DNA Methylation Fibroblasts Full Papers Long terminal repeat CpG site Gene Expression Regulation DNA methylation embryonic structures CpG Islands Female HERV methylation |
| Zdroj: | DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes |
| ISSN: | 1756-1663 |
| Popis: | Human endogenous retroviruses (HERVs) are globally silent in somatic cells. However, some HERVs display high transcription in physiological conditions. In particular, ERVWE1, ERVFRDE1 and ERV3, three proviruses of distinct families, are highly transcribed in placenta and produce envelope proteins associated with placenta development. As silencing of repeated elements is thought to occur mainly by DNA methylation, we compared the methylation of ERVWE1 and related HERVs to appreciate whether HERV methylation relies upon the family, the integration site, the tissue, the long terminal repeat (LTR) function or the associated gene function. CpG methylation of HERV-W LTRs in placenta-associated tissues was heterogeneous but a joint epigenetic control was found for ERVWE1 5'LTR and its juxtaposed enhancer, a mammalian apparent LTR retrotransposon. Additionally, ERVWE1, ERVFRDE1 and ERV3 5'LTRs were all essentially hypomethylated in cytotrophoblasts during pregnancy, but showed distinct and stage-dependent methylation profiles. In non-cytotrophoblastic cells, they also exhibited different methylation profiles, compatible with their respective transcriptional activities. Comparative analyses of transcriptional activity and LTR methylation in cell lines further sustained a role for methylation in the control of functional LTRs. These results suggest that HERV methylation might not be family related but copy-specific, and related to the LTR function and the tissue. In particular, ERVWE1 and ERV3 could be developmentally epigenetically regulated HERVs. |
| Databáze: | OpenAIRE |
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