Cochlear outer hair cells undergo an apical circumference remodeling constrained by the hair bundle shape
| Autor: | Christine Petit, Dominique Weil, Michel Leibovici, Vincent Michel, Isabelle Foucher, Nadège Cayet, Raphaël Etournay, Jean-Pierre Hardelin, Jacques Boutet de Monvel, Léa Lepelletier |
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| Přispěvatelé: | Etournay, Raphael, Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Max-Planck-Gesellschaft, Collège de France - Chaire Génétique et physiologie cellulaire, Collège de France (CdF (institution)), Université Paris Diderot - Paris 7 (UPD7), Microscopie Ultrastructurale (Plate-forme), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Département de Biologie du Développement, Institut Pasteur [Paris] (IP), Université Paris-Sud - Paris 11 (UP11), Développement, évolution et plasticité du système nerveux (DEPSN), Centre National de la Recherche Scientifique (CNRS), R.E. and L.L. received fellowships from the Collège de France and the Fondation pour la Recherche Médicale, respectively, R.E. acknowledges a Marie Curie fellowship from the EU 7th Framework Programme (FP7), J.B.M. acknowledges support from the Hugot Foundation of the Collège de France.This work was supported by grants from the Fondation Raymonde et Guy Strittmatter, the Réunica-Prévoyance Group, European Commission FP6 Integrated Project EuroHear (LSHG CT-2004-512063), the A. & M. Suchert-Retina Kontra Blindheit Stiftung and the French National Research Agency., Chaire Génétique et physiologie cellulaire, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] |
| Rok vydání: | 2010 |
| Předmět: |
MESH: Neurons
MESH: Hair Cells Auditory Outer Cell junction Mice 0302 clinical medicine MESH: Cochlea Myosin Scanning MESH: Animals MESH: Organ of Corti Cytoskeleton Auditory Organ of Corti Neurons Microscopy 0303 health sciences Goats Cilium Ear Anatomy Cochlea Cell biology Hair Cells medicine.anatomical_structure Mechanosensitive channels MESH: Microscopy Electron Scanning MESH: Microscopy Electron [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Outer Electron MESH: Goats 03 medical and health sciences MESH: Cilia medicine Animals Cilia [SDV.BC] Life Sciences [q-bio]/Cellular Biology MESH: Mice Molecular Biology 030304 developmental biology Apical constriction Inner Hair Cells Auditory Outer Microscopy Electron Ear Inner Microscopy Electron Scanning sense organs MESH: Ear Inner 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Development (Cambridge, England) Development (Cambridge, England), 2010, 137 (8), pp.1373-83. ⟨10.1242/dev.045138⟩ Development (Cambridge, England), Company of Biologists, 2010, 137 (8), pp.1373-83. ⟨10.1242/dev.045138⟩ |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.045138 |
| Popis: | International audience; Epithelial cells acquire diverse shapes relating to their different functions. This is particularly relevant for the cochlear outer hair cells (OHCs), whose apical and basolateral shapes accommodate the functioning of these cells as mechano-electrical and electromechanical transducers, respectively. We uncovered a circumferential shape transition of the apical junctional complex (AJC) of OHCs, which occurs during the early postnatal period in the mouse, prior to hearing onset. Geometric analysis of the OHC apical circumference using immunostaining of the AJC protein ZO1 and Fourier-interpolated contour detection characterizes this transition as a switch from a rounded-hexagon to a non-convex circumference delineating two lateral lobes at the neural side of the cell, with a negative curvature in between. This shape tightly correlates with the 'V'-configuration of the OHC hair bundle, the apical mechanosensitive organelle that converts sound-evoked vibrations into variations in cell membrane potential. The OHC apical circumference remodeling failed or was incomplete in all the mouse mutants affected in hair bundle morphogenesis that we tested. During the normal shape transition, myosin VIIa and myosin II (A and B isoforms) displayed polarized redistributions into and out of the developing lobes, respectively, while Shroom2 and F-actin transiently accumulated in the lobes. Defects in these redistributions were observed in the mutants, paralleling their apical circumference abnormalities. Our results point to a pivotal role for actomyosin cytoskeleton tensions in the reshaping of the OHC apical circumference. We propose that this remodeling contributes to optimize the mechanical coupling between the basal and apical poles of mature OHCs. |
| Databáze: | OpenAIRE |
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