Diets high in fermentable protein and fibre alter tight junction protein composition with minor effects on barrier function in piglet colon
| Autor: | Dorothee Günzel, Silke S. Zakrzewski, Robert Pieper, Joerg D. Schulzke, Jan F. Richter, Andrew G. Van Kessel |
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| Rok vydání: | 2013 |
| Předmět: |
Dietary Fiber
Male medicine.medical_specialty Colon Sus scrofa Medicine (miscellaneous) Occludin Horseradish peroxidase Internal medicine Submucosa medicine Animals Intestinal Mucosa Ion transporter Barrier function Nutrition and Dietetics Tight Junction Proteins biology Tight junction Chemistry Diet medicine.anatomical_structure Endocrinology Biochemistry Intestinal Absorption Permeability (electromagnetism) Paracellular transport Fermentation biology.protein Female Dietary Proteins |
| Zdroj: | The British journal of nutrition. 111(6) |
| ISSN: | 1475-2662 |
| Popis: | Protein fermentation end products may damage the colonic mucosa, which could be counteracted by dietary inclusion of fermentable carbohydrates (fCHO). Although fermentable crude protein (fCP) and fCHO are known to affect microbial ecology, their interactive effects on epithelial barrier function are unknown. In the present study, in a 2 × 2 factorial experiment, thirty-two weaned piglets were fed low-fCP/low-fCHO (14·5 % crude protein (CP)/14·5 % total dietary fibre (TDF)), low-fCP/high-fCHO (14·8 % CP/16·6 % TDF), high-fCP/low-fCHO (19·8 % CP/14·5 % TDF) and high-fCP/high-fCHO (20·1 % CP/18·0 % TDF) diets. After 21–23 d, samples of proximal and distal colonic mucosae were investigated in Ussing chambers with respect to the paracellular and transcytotic passages of macromolecules and epithelial ion transport. The high-fCHO diets were found to reduce the permeability of the distal colon to the transcytotic marker horseradish peroxidase (HRP, 44 kDa;P <0·05) and also reduce the paracellular permeation ofN-hydroxysuccinimide-biotin into the submucosa (443 Da;P <0·05), whereas that of HRP was decreased by the high-fCP diets (P <0·01). Short-circuit current (active ion transport), transepithelial resistance (barrier function) and charge selectivity were largely unaffected in both the segments. However, the high-fCP diets were found to suppress the aldosterone-induced epithelial Na channel activity (P <0·01) irrespective of fCHO inclusion. The high-fCP diets generally reduced the expression of colonic claudin-1, claudin-2 and claudin-3 (P <0·01), while that of claudin-4 was increased by the high-fCHO diets (P <0·01). The high-fCHO diets also altered the ratio between occludin forms (P <0·05) and increased the expression of tricellulin in the proximal colon, which was not observed with high-fCP diets. In conclusion, dietary fCHO and fCP exerted few and largely independent effects on functional measurements, but altered tight junction protein composition in a compensatory way, so that colonic transport and barrier properties were only marginally affected. |
| Databáze: | OpenAIRE |
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