An assessment of the impact of green tea extract on palbociclib pharmacokinetics using a validated UHPLC‐QTOF‐MS method
| Autor: | Patheparapu Chandrakala, David Paul, N. Satheeshkumar, Shruthi Surendran |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Pyridines Formic acid Clinical Biochemistry Herb-Drug Interactions Green tea extract Palbociclib 01 natural sciences Biochemistry High-performance liquid chromatography Piperazines Analytical Chemistry Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Drug Stability Pharmacokinetics Limit of Detection Drug Discovery Animals Protein precipitation Sample preparation Molecular Biology Chromatography High Pressure Liquid Pharmacology Chromatography Tea Plant Extracts Chemistry 010401 analytical chemistry Reproducibility of Results General Medicine Rats 0104 chemical sciences Bioavailability 030104 developmental biology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Linear Models |
| Zdroj: | Biomedical Chromatography. :e4469 |
| ISSN: | 1099-0801 0269-3879 |
| DOI: | 10.1002/bmc.4469 |
| Popis: | Green tea extracts (GTE) has been reported to be a kinase inhibitor and modulator for various drug metabolizing enzymes. It may give synergetic antioncogenic effect, but with a possibility of pharmacokinetic interactions with various co-administered anticancer agents like palbociclib (PAL), a selective inhibitor of CDK-4/6 primarily metabolized by CYP3A enzyme. To explore the impact of GTE on PAL pharmacokinetics in Sprague-Dawley rats, a rapid and sensitive UHPLC-QTOF-MS method was established. Chromatographic separation was carried out on an Acquity UPLC BEH C18 (100 × 2.1 mm, 1.7 μm) column using a gradient mobile phase system consisting of 0.1% formic acid and acetonitrile. Sample preparation was based on a simple protein precipitation method. Estimation of target ions [M + H]+ at m/z 448.2455 for PAL and m/z 441.2044 for ibrutinib (IS) was performed in selective ion mode ESI-HRMS. Good sensitivity (1.0 ng/mL) and linearity over a wide concentration range of 1-2000 ng/mL was exhibited by the method. The results indicated that the administration of GTE resulted in decreased oral bioavailability of PAL in both short- and long-term conditions. However, when both conditions were compared, the variation was less for the peak concentration and area under the concentration-time curve level of PAL. |
| Databáze: | OpenAIRE |
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