Regulation of mammalian MOR-1 gene expression after chronic treatment with morphine
| Autor: | Rose V. Prenus, Carl B. Goodman, Ebens Luscar, Zhi-Ping Zhu, Ramesh B. Badisa |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Narcotic Antagonists Receptors Opioid mu CHO Cells (+)-Naloxone Biology Pharmacology 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Line Tumor Cricetinae Internal medicine Gene expression Genetics medicine Animals Humans RNA Messenger 030304 developmental biology Regulation of gene expression 0303 health sciences Morphine naloxone Chinese hamster ovary cell Articles General Medicine Transfection real-time qRT-PCR Analgesics Opioid Endocrinology Gene Expression Regulation Opioid Cell culture μ-opioid receptor 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| Popis: | Morphine is an effective analgesic that acts by binding to the μ-opioid receptor (MOR) coded in the human by the OPRM1 gene. In the present study, we investigated the regulation of μ-opioid receptor (MOR-1) mRNA levels in all-trans-retinoic acid-differentiated SH-SY5Y human neuroblastoma cells under in vitro conditions with 10 μM morphine treatment for 24 h. In addition, we measured the MOR-1 levels in recombinant Chinese hamster ovary (CHO) cells, transfected with human μ-opioid receptor gene (hMOR) with 10 μM morphine treatment for 24 h. The isolated mRNA from these cells was subjected to real-time quantitative RT-PCR analysis to determine the regulation of μ-opioid receptor gene expression. It was observed that morphine treatment did not alter MOR-1 levels in undifferentiated SH-SY5Y cells compared to undifferentiated control cells. However, the MOR-1 levels in all-trans-retinoic acid-differentiated cells were significantly higher compared to the undifferentiated cells. Morphine treatment in differentiated SH-SY5Y cells caused significant downregulation of MOR-1 expression compared to the control cells. In the morphine-treated CHO cells, the hMOR-1 mRNA levels remained the same as the untreated control. Finally, pretreatment of SH-SY5Y cells with 10 μM naloxone, the antagonist of μ-opioid receptor, for 1 h significantly blocked the downregulation of MOR-1 mRNA levels with morphine treatment. These findings suggest that regulation of MOR-1 gene expression is cell-type specific after chronic morphine treatment and provide some evidence in the understanding of morphine tolerance. |
| Databáze: | OpenAIRE |
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