Neurons with histaminelike immunoreactivity in the segmental and stomatogastric nervous systems of the crayfishPacifastacus leniusculus and the lobsterHomarus americanus
| Autor: | Brian Mulloney, Wendy M. Hall |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Histology
Interneuron Astacoidea Pathology and Forensic Medicine Esophagus Interneurons Stomatogastric nervous system medicine Neuropil Animals Thoracic ganglia Brain Chemistry Neurotransmitter Agents Homarus biology musculoskeletal neural and ocular physiology Cell Biology Anatomy Thorax Stomatogastric ganglion biology.organism_classification Crayfish Immunohistochemistry Nephropidae Ganglion medicine.anatomical_structure nervous system Ganglia sense organs Neuroscience Histamine |
| Zdroj: | Cell and Tissue Research. 266:197-207 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/bf00678725 |
| Popis: | We used a polyclonal antiserum against histamine to map histaminelike immunoreactivity (HLI) in whole mounts of the segmental ganglia and stomatogastric ganglion of crayfish and lobster. Carbodiimide fixation permitted both HRP-conjugated and FITC-conjugated secondary antibodies to be used effectively to visualize HLI in these whole mounts. Two interneurons that send axons through the inferior ventricular nerve (ivn) and the stomatogastric nerve to the stomatogastric ganglion had strong HLI, both in crayfish and in lobster. These ivn interneurons were known from other evidence to be histaminergic. The neuropil of the stomatogastric ganglion in both crayfish and lobster contained brightly labeled terminals of axons that entered the ganglion from the stomatogastric nerve. No neuronal cell bodies in this ganglion had HLI. Each segmental ganglion contained at least one pair of neurons with HLI. Some neurons in the subesophageal ganglion and in each thoracic ganglion labeled very brightly. Axons of projection interneurons with strong HLI occurred in the dorsal lateral tracts of each segmental ganglion, and sent branches to the lateral neurophils and tract neurophils of each ganglion. All the labeled neurons were interneurons; no HLI was observed in peripheral nerves. |
| Databáze: | OpenAIRE |
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