In-Vivo Optical Monitoring of the Efficacy of Epidermal Growth Factor Receptor Targeted Photodynamic Therapy
| Autor: | Max J. H. Witjes, Go M. van Dam, Dominic J. Robinson, Jan L. N. Roodenburg, Henriëtte S. de Bruijn, Wei Peng, Timo L.M. ten Hagen, Kristian Berg |
|---|---|
| Přispěvatelé: | Damage and Repair in Cancer Development and Cancer Treatment (DARE), Guided Treatment in Optimal Selected Cancer Patients (GUTS), Microbes in Health and Disease (MHD), Basic and Translational Research and Imaging Methodology Development in Groningen (BRIDGE), Otorhinolaryngology and Head and Neck Surgery, Surgery, Erasmus MC other |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research photosensitizer ILLUMINATION medicine.medical_treatment EGFR chemistry.chemical_element Photodynamic therapy SCATTERING PROPERTIES lcsh:RC254-282 Oxygen Article TUMOR OXYGENATION 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine growth delay intravital microscopy medicine polycyclic compounds Photosensitizer Epidermal growth factor receptor HEAD targeted biology Chemistry Singlet oxygen FRACTIONATION PHOTOIMMUNOTHERAPY DIAMETER Photoimmunotherapy QUANTIFICATION lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Photobleaching CANCER OXYGEN-CONSUMPTION 030104 developmental biology Oncology photodynamic therapy 030220 oncology & carcinogenesis Biophysics biology.protein Sodium azide |
| Zdroj: | Cancers, 12(1):190. MDPI AG Cancers Volume 12 Issue 1 Cancers, 12. Multidisciplinary Digital Publishing Institute (MDPI) Cancers, Vol 12, Iss 1, p 190 (2020) |
| ISSN: | 2072-6694 |
| Popis: | Targeted photodynamic therapy (PDT) has the potential to improve the therapeutic effect of PDT due to significantly better tumor responses and less normal tissue damage. Here we investigated if the efficacy of epidermal growth factor receptor (EGFR) targeted PDT using cetuximab-IRDye700DX is fluence rate dependent. Cell survival after treatment with different fluence rates was investigated in three cell lines. Singlet oxygen formation was investigated using the singlet oxygen quencher sodium azide and singlet oxygen sensor green (SOSG). The long-term response (to 90 days) of solid OSC-19-luc2-cGFP tumors in mice was determined after illumination with 20, 50, or 150 mW· cm&minus 2. Reflectance and fluorescence spectroscopy were used to monitor therapy. Singlet oxygen was formed during illumination as shown by the increase in SOSG fluorescence and the decreased response in the presence of sodium azide. Significantly more cell death and more cures were observed after reducing the fluence rate from 150 mW· 2 to 20 mW· 2 both in-vitro and in-vivo. Photobleaching of IRDye700DX increased with lower fluence rates and correlated with efficacy. The response in EGFR targeted PDT is strongly dependent on fluence rate used. The effectiveness of targeted PDT is, like PDT, dependent on the generation of singlet oxygen and thus the availability of intracellular oxygen. |
| Databáze: | OpenAIRE |
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