Dynamin A, Myosin IB and Abp1 Couple Phagosome Maturation to F-Actin Binding
| Autor: | Régis Dieckmann, Eik Hoffmann, Katja Hille, Thierry Soldati, Roger Monroy, Devang M. Patel, Eva M. Neuhaus, Monica Hagedorn, Sergei A. Kuznetsov, Navin Andréw Gopaldass, Julia Krüger, Stéphanie Hausherr, Ramona Kratzke |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Dynamins
Endosome Phagosome acidification Blotting Western Endocytic cycle Protozoan Proteins Endosomes macromolecular substances Biology Models Biological Biochemistry Gene Knockout Techniques Myosin Type I 03 medical and health sciences 0302 clinical medicine Phagocytosis Structural Biology Phagosomes Myosin Phagosome maturation Genetics Dictyostelium Molecular Biology Actin 030304 developmental biology Phagosome Dynamin 0303 health sciences Microfilament Proteins Cell Biology Actins Cell biology Protein Transport ddc:540 030217 neurology & neurosurgery |
| Zdroj: | Traffic, Vol. 13, No 1 (2012) pp. 120-30 Traffic (Copenhagen Denmark) |
| ISSN: | 1398-9219 |
| DOI: | 10.1111/j.1600-0854.2011.01296.x |
| Popis: | The role of actin, class I myosins and dynamin in endocytic uptake processes is well characterized, but their role during endo-phagosomal membrane trafficking and maturation is less clear. In Dictyostelium, knockout of myosin IB (myoB) leads to a defect in membrane protein recycling from endosomes back to the plasma membrane. Here, we show that actin plays a central role in the morphology and function of the endocytic pathway. Indeed, latrunculin B (LatB) induces endosome tubulation, a phenotype also observed in dynamin A (dymA)-null cells. Knockout of dymA impairs phagosome acidification, whereas knockout of myoB delays reneutralization, a phenotype mimicked by a low dose of LatB. As a read out for actin-dependent processes during maturation, we monitored the capacity of purified phagosomes to bind F-actin in vitro, and correlated this with the presence of actin-binding and membrane-trafficking proteins. Phagosomes isolated from myoB-null cells showed an increased binding to F-actin, especially late phagosomes. In contrast, early phagosomes from dymA-null cells showed reduced binding to F-actin while late phagosomes were unaffected. We provide evidence that Abp1 is the main F-actin-binding protein in this assay and is central for the interplay between DymA and MyoB during phagosome maturation. |
| Databáze: | OpenAIRE |
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