Multispectral imaging in the extended near-infrared window based on endogenous chromophores
| Autor: | Qian Cao, Mikhail Y. Berezin, Walter J. Akers, Steven T. Wang, Natalia G. Zhegalova |
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| Rok vydání: | 2013 |
| Předmět: |
Materials science
Multispectral image Biomedical Engineering Mice Nude Models Biological Biomaterials Absorbance Mice Optics Image Processing Computer-Assisted Animals Penetration depth Spectroscopy Absorption (electromagnetic radiation) Spectroscopy Near-Infrared Scattering business.industry Near-infrared spectroscopy Optical Imaging Special Section Papers Water Lipids Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Autofluorescence Optoelectronics Female business Head |
| Zdroj: | Journal of biomedical optics. 18(10) |
| ISSN: | 1560-2281 |
| Popis: | To minimize the problem with scattering in deep tissues while increasing the penetration depth, we explored the feasibility of imaging in the relatively unexplored extended near infrared (exNIR) spectral region at 900 to 1400 nm with endogenous chromophores. This region, also known as the second NIR window, is weakly dominated by absorption from water and lipids and is free from other endogenous chromophores with virtually no autofluorescence. To demonstrate the applicability of the exNIR for bioimaging, we analyzed the optical properties of individual components and biological tissues using an InGaAs spectrophotometer and a multispectral InGaAs scanning imager featuring transmission geometry. Based on the differences in spectral properties of tissues, we utilized ratiometric approaches to extract spectral characteristics from the acquired three-dimensional “datacube”. The obtained images of an exNIR transmission through a mouse head revealed sufficient details consistent with anatomical structures. |
| Databáze: | OpenAIRE |
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