Mast Cells Migrate from Blood to Brain
| Autor: | Marta Wilhelm, Anne K. Sutherland, Rae Silver, Ann Judith Silverman |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Pathology
medicine.medical_specialty Population Fluorescent Antibody Technique Blood–brain barrier chemistry.chemical_compound Cell Movement Parenchyma medicine Animals Mast Cells ARTICLE Rats Wistar education Fluorescent Dyes Aldehydes education.field_of_study Microscopy Confocal Glial fibrillary acidic protein biology General Neuroscience Age Factors Brain Mast cell Rats Haematopoiesis medicine.anatomical_structure chemistry Blood-Brain Barrier biology.protein Female Basal lamina Histamine |
| Zdroj: | The Journal of Neuroscience. 20:401-408 |
| ISSN: | 1529-2401 0270-6474 |
| Popis: | It is well established that mast cells (MCs) occur within the CNS of many species. Furthermore, their numbers can increase rapidly in adults in response to altered physiological conditions. In this study we found that early postpartum rats had significantly more mast cells in the thalamus than virgin controls. Evidence from semithin sections from these females suggested that mast cells were transiting across the medium-sized blood vessels. We hypothesized that the increases in mast cell number were caused by their migration into the neural parenchyma. To this end, we purified rat peritoneal mast cells, labeled them with the vital dyes PKH26 or CellTracker Green, and injected them into host animals. One hour after injection, dye-filled cells, containing either histamine or serotonin (mediators stored in mast cells), were located close to thalamic blood vessels. Injected cells represented ∼2–20% of the total mast cell population in this brain region. Scanning confocal microscopy confirmed that the biogenic amine and the vital dye occurred in the same cell. To determine whether the donor mast cells were within the blood–brain barrier, we studied the localization of dye-marked donor cells and either Factor VIII, a component of endothelial basal laminae, or glial fibrillary acidic protein, the intermediate filament found in astrocytes. Serial section reconstructions of confocal images demonstrated that the mast cells were deep to the basal lamina, in nests of glial processes. This is the first demonstration that mast cells can rapidly penetrate brain blood vessels, and this may account for the rapid increases in mast cell populations after physiological manipulations. |
| Databáze: | OpenAIRE |
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