Roles of epithelial cell-derived periostin in TGF-β activation, collagen production, and collagen gel elasticity in asthma
| Autor: | Sheena C. Kerr, Lydia Hou, Sukhvinder Sidhu, Shaopeng Yuan, Prescott G. Woodruff, Susan J. Muller, John V. Fahy, Anh L. Innes |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Bronchi
Matrix metalloproteinase Periostin Collagen Type I Transforming Growth Factor beta medicine Humans Cells Cultured Multidisciplinary Chemistry Matricellular protein Mesenchymal stem cell Fibrillogenesis Epithelial Cells Biological Sciences Epithelium Asthma Elasticity Matrix Metalloproteinases Cell biology medicine.anatomical_structure Immunology Signal transduction Cell Adhesion Molecules Gels Extracellular matrix organization |
| Popis: | Periostin is considered to be a matricellular protein with expression typically confined to cells of mesenchymal origin. Here, by using in situ hybridization, we show that periostin is specifically up-regulated in bronchial epithelial cells of asthmatic subjects, and in vitro, we show that periostin protein is basally secreted by airway epithelial cells in response to IL-13 to influence epithelial cell function, epithelial–mesenchymal interactions, and extracellular matrix organization. In primary human bronchial epithelial cells stimulated with periostin and epithelial cells overexpressing periostin, we reveal a function for periostin in stimulating the TGF-β signaling pathway in a mechanism involving matrix metalloproteinases 2 and 9. Furthermore, conditioned medium from the epithelial cells overexpressing periostin caused TGF-β–dependent secretion of type 1 collagen by airway fibroblasts. In addition, mixing recombinant periostin with type 1 collagen in solution caused a dramatic increase in the elastic modulus of the collagen gel, indicating that periostin alters collagen fibrillogenesis or cross-linking and leads to stiffening of the matrix. Epithelial cell-derived periostin in asthma has roles in TGF-β activation and collagen gel elasticity in asthma. |
| Databáze: | OpenAIRE |
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