Assessment of bacterial inoculant formulated with Paraburkholderia tropica to enhance wheat productivity
| Autor: | Ana Clara López, Sabrina Soledad García, Santiago Adolfo Vio, María Flavia Luna, Pamela Romina Bernabeu, José Luis Boiardi, Natalia Carrasco |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Physiology Nitrogen Biofertilizer Biología Biotecnología Agropecuaria 030106 microbiology Biology Applied Microbiology and Biotechnology Plant Roots Seed treatment Paraburkholderia Crop 03 medical and health sciences chemistry.chemical_compound Nitrogen Fixation Endophytes Colonization Microbial inoculant Soil Microbiology Triticum Ciencias Exactas Rhizosphere Inoculation Burkholderiaceae Crop yield food and beverages General Medicine Agricultural Inoculants Crop Production Horticulture 030104 developmental biology chemistry CIENCIAS AGRÍCOLAS Wheat Biotecnología Agrícola y Biotecnología Alimentaria Plant growth promotion Biotechnology |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| Popis: | Paraburkholderia tropica is an endophytic nitrogen-fixing bacterium isolated from the rhizosphere, rhizoplane, and internal tissues of sugarcane and corn plants in different geographical regions. Other plant-growth-promoting abilities, such as phosphate solubilization and antifungal activity, have also been reported for this bacterium. With an aim at investigating the potential use of P. tropica as an inoculant for improving the performance of wheat crop, in this work we evaluated an experimental inoculant formulated with P. tropica MTo-293 with respect to root colonization, the practical aspects of its application, and the effects under field conditions when applied to wheat seeds. Bacterial colonization was monitored by culture dependent techniques and the wheat yield determined by quantifying the total grain production in two different seasons. Rhizoplane and endophytic colonization in wheat roots was achieved efficiently (on average, 8 and 4 log colony-forming units/g fresh weight, respectively) even at relatively low concentrations of viable bacteria in the inoculum under controlled conditions. P. tropica was compatible with a widely used fungicide, maintained viability for 48 h once applied to seeds, and was also able to colonize wheat roots efficiently. Furthermore, we were able to formulate an inoculant that maintained bacterial viability for relatively long time periods. Preliminary field assays were realized, and even though the average yields values for the inoculated treatments remained above the uninoculated ones, no significant effects of inoculation were detected with or without fertilization. The correct physiologic behavior of P. tropica suggests the necessity to continue with field experiments under different conditions. Centro de Investigación y Desarrollo en Fermentaciones Industriales |
| Databáze: | OpenAIRE |
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