MERS versus Standard Surgical Approaches for Porcine Vocal Fold Scarring with Adipose Stem Cell Constructs
Autor: | Henry T. Hoffman, Joo Hyun Woo, Suzanne N. King, Michael B. Christensen, Susan L. Thibeault, Seth H. Dailey, Sarah Wang |
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Rok vydání: | 2016 |
Předmět: |
Pathology
medicine.medical_specialty Swine Basic fibroblast growth factor Adipose tissue Fibrin Tissue Adhesive Vocal Cords Matrix metalloproteinase Real-Time Polymerase Chain Reaction Article Immunoenzyme Techniques Cicatrix 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals 030223 otorhinolaryngology Cells Cultured Lamina propria Laryngoscopy biology business.industry Hydrogels Fibronectin Disease Models Animal medicine.anatomical_structure Otorhinolaryngology chemistry 030220 oncology & carcinogenesis biology.protein Female Surgery Hepatocyte growth factor Stem cell business Biomarkers Stem Cell Transplantation medicine.drug Transforming growth factor |
Zdroj: | Otolaryngology–Head and Neck Surgery. 155:612-623 |
ISSN: | 1097-6817 0194-5998 |
Popis: | OBJECTIVE. Cells, scaffold, and surgical approaches are important for regeneration of the lamina propria of the scarred vocal fold (VF). Microendoscopy of Reinke’s space (MERS) is a surgical approach used to access the lamina propria. The present study evaluated MERS in the treatment of VF scarring as compared with standardized approaches for the treatment of VF scarring with adipose stem cell constructs. STUDY DESIGN. Animal study. SETTING. Academic center. SUBJECTS AND METHODS. VF injury was performed bilaterally to induce scarring in 20 pigs. Eight weeks after injury, pigs were classified into no treatment, minithyrotomy, VF injection, VF incision/dissection, and MERS. All groups (except control) were implanted with adipose stem cell and hyaluronan. Four weeks after treatment, histology for collagen, hyaluronan, and fibronectin; mRNA expression for α-smooth muscle actin, tumor growth factor β1, collagen 1α1, collagen 3α1, matrix metalloproteinase 2, basic fibroblast growth factor, and hepatocyte growth factor; and tissue rheology were evaluated. RESULTS. Differences were measured among surgical approaches for protein levels of collagen, hyaluronan, and fibronectin (P = .0133, P < .0001, and P = .0025, respectively). Fibroblast growth factor, collagen 1α1, and matrix metalloproteinase 2 transcript levels were different among treatment groups (P = .003, P = .0086, and P = .014, respectively), while no differences were measured for α-smooth muscle actin, tumor growth factor β1, hepatocyte growth factor, and collagen 3α1. Rheologically, significant differences were not measured between groups. CONCLUSION. MERS is a promising surgical approach for the treatment of VF scarring, optimizing the placement of implanted biomaterials. |
Databáze: | OpenAIRE |
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