Selection and Characterization of Anti-Dengue NS1 Single Domain Antibodies
| Autor: | Cheng-Rei Lee, Shuenn-Jue L. Wu, Lisa C. Shriver-Lake, Victor A. Sugiharto, George M. Anderson, Ellen R. Goldman, Dan Zabetakis, Gabriel Defang, Jinny L. Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Phage display viruses lcsh:Medicine Biology Dengue virus Viral Nonstructural Proteins medicine.disease_cause Antibodies Viral Cross-reactivity Virus Article Dengue fever Zika virus 03 medical and health sciences 0302 clinical medicine Antigen medicine Humans Amino Acid Sequence lcsh:Science Multidisciplinary Spectrum Analysis lcsh:R virus diseases Japanese encephalitis biochemical phenomena metabolism and nutrition Dengue Virus Single-Domain Antibodies Surface Plasmon Resonance medicine.disease biology.organism_classification Virology 030104 developmental biology 030220 oncology & carcinogenesis lcsh:Q |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
| ISSN: | 2045-2322 |
| Popis: | Reliable detection and diagnosis of dengue virus (DENV) is important for both patient care and epidemiological control. Starting with a llama immunized with a mixture of recombinant nonstructural protein 1 (NS1) antigen from the four DENV serotypes, a phage display immune library of single domain antibodies was constructed and binders selected which exhibited specificity and affinity for DENV NS1. Each of these single domain antibodies was evaluated for its binding affinity to NS1 from the four serotypes, and incorporated into a sandwich format for NS1 detection. An optimal pair was chosen that provided the best combination of sensitivity for all four DENV NS1 antigens spiked into 50% human serum while showing no cross reactivity to NS1 from Zika virus, yellow fever virus, tick-borne encephalitis virus, and minimal binding to NS1 from Japanese encephalitis virus and West Nile virus. These rugged and robust recombinant binding molecules offer attractive alternatives to conventional antibodies for implementation into immunoassays destined for resource limited locals. |
| Databáze: | OpenAIRE |
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