The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro
| Autor: | Cristina Canal, Jean-Sébastien Thomann, Maïté Audemar, Piotr Terebun, Agata Przekora, Grazyna Ginalska, Joanna Pawłat, David Duday, Sophie Hermans, Cédric Labay |
|---|---|
| Přispěvatelé: | UCL - SST/IMCN - Institute of Condensed Matter and Nanosciences, Universitat Politècnica de Catalunya. Departament de Ciència i Enginyeria de Materials, Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits, Medical University of Lublin, Lublin University of Technology, Luxembourg Institute of Science and Technology, Université Catholique de Louvain, Uniwersytet Medyczny w Lublinie, Politechnika Lubelska |
| Rok vydání: | 2019 |
| Předmět: |
Cell viability
ECM mineralization Acoustics and Ultrasonics Phosphatase GlidArc Enginyeria dels materials [Àrees temàtiques de la UPC] Coatings and Films Plasma Plasma activated medium Electronic Viability assay Optical and Magnetic Materials chemistry.chemical_classification Chemistry Mc3t3 e1 Condensed Matter Physics Skeleton (computer programming) In vitro Surfaces Coatings and Films Electronic Optical and Magnetic Materials Plasma activated cells Surfaces Enzyme Biochemistry Toxicity Alkaline phosphatase |
| Zdroj: | JOURNAL OF PHYSICS D-APPLIED PHYSICS r-FSJD: Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu Fundació Sant Joan de Déu Journal of Physics D: Applied Physics, Vol. 52, no.27, p. 275401 (2019) Recercat. Dipósit de la Recerca de Catalunya instname UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu |
| ISSN: | 0022-3727 |
| Popis: | The aim of this work was to evaluate the impact of atmospheric pressure nitrogen plasma on viability, proliferation, and osteogenic differentiation of normal mouse calvarial preosteoblasts (MC3T3-E1 Subclone 4), which were maintained in Hanks’ balanced salt solution (HBSS) during plasma exposure. Obtained results clearly demonstrated that short-time (4, 8, and 16 s) nitrogen plasma treatment is non-toxic to the MC3T3-E1 cells, does not affect cell morphology, promotes preosteoblasts’ proliferation, enhances osteogenic differentiation by increasing bone alkaline phosphatase and osteocalcin concentration, but inhibits mineralization of extracellular matrix. The best results were achieved for 16 s exposure time and when the preosteoblasts were left in HBSS for 3 h after plasma treatment. Presented studies indicate great clinical potential of cold atmospheric nitrogen plasma for regenerative medicine applications to improve bone healing process. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|