Additive Manufacturing of Bovine Serum Albumin-Based Hydrogels and Bioplastics
Autor: | Jonathan H. Tsui, Patrick T. Smith, Deok Ho Kim, Benjaporn Narupai, S. Cem Millik, Ryan T. Shafranek, Alshakim Nelson |
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Rok vydání: | 2019 |
Předmět: |
Stereolithography
Fabrication Materials science Polymers and Plastics Cell Survival 3D printing Bioengineering 02 engineering and technology 010402 general chemistry Methacrylate 01 natural sciences Bioplastic Polyethylene Glycols Polymerization law.invention Biomaterials Mice law Materials Testing Organometallic Compounds Materials Chemistry Animals Bovine serum albumin Rheometry biology Viscosity business.industry Circular Dichroism technology industry and agriculture Hydrogels Serum Albumin Bovine 021001 nanoscience & nanotechnology Biodegradable polymer 0104 chemical sciences Resins Synthetic Cross-Linking Reagents Photopolymer Chemical engineering Printing Three-Dimensional Self-healing hydrogels NIH 3T3 Cells biology.protein Methacrylates business 0210 nano-technology Plastics |
Zdroj: | Biomacromolecules. 21:484-492 |
ISSN: | 1526-4602 1525-7797 |
Popis: | Bio-sourced and biodegradable polymers for additive manufacturing could enable the rapid fabrication of parts for a broad spectrum of applications ranging from healthcare to aerospace. However, a limited number of these materials are suitable for vat photopolymerization processes. Herein, we report a two-step additive manufacturing process to fabricate robust protein-based constructs using a commercially available laser-based SLA printer. Methacrylated bovine serum albumin (MA-BSA) was synthesized and formulated into aqueous resins that were used to print complex 3D objects with a resolution comparable to a commercially available resin. The MA-BSA resins were characterized by rheometry to determine the viscosity and the cure rate, as both of these parameters can ultimately be used to predict the printability of the resin. In the first step of patterning these materials, the MA-BSA resin was 3D printed, and in the second step, the printed construct was thermally cured to denature the globular protein and increase the intermolecular noncovalent interactions. Thus, the final 3D printed part was comprised of both chemical and physical cross-links. Compression studies of hydrated and dehydrated constructs demonstrated a broad range of compressive strengths and Young’s moduli that could be further modulated by adjusting the type and amount of co-monomer. The printed hydrogel constructs demonstrated good cell viability (> 95%) after a 21-day culture period. These MA-BSA resins are expected to be compatible with other vat photopolymerization techniques including digital light projection (DLP) and continuous liquid interface production (CLIP). |
Databáze: | OpenAIRE |
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