Ontogeny of Bradykinin B1 Receptors in the Mouse Kidney
| Autor: | Susana Dipp, Samir S. El-Dahr, Ozlem Pinar Bulut |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Time Factors Mesenchyme Neuropeptide Bradykinin Inflammation Nephron Biology Kidney Receptor Bradykinin B1 Article Receptors G-Protein-Coupled Mice chemistry.chemical_compound Internal medicine medicine Animals Kidney Tubules Collecting Receptor Mice Knockout Reverse Transcriptase Polymerase Chain Reaction Embryogenesis Gene Expression Regulation Developmental Nephrons Fibrosis medicine.anatomical_structure Endocrinology Microscopy Fluorescence chemistry Pediatrics Perinatology and Child Health medicine.symptom Peptides |
| Zdroj: | Pediatric Research. 66:519-523 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/pdr.0b013e3181b4f8c2 |
| Popis: | Kinins are vasoactive peptides that stimulate two G protein coupled bradykinin receptors (B1R and B2R). B2R-knockout (KO) mice are salt-sensitive and develop renal dysgenesis and hypertension if salt-stressed during embryogenesis. B1R-KO mice, on the other hand, are protected from inflammation and fibrosis. The present study examined the spatiotemporal expression of B1R during renal organogenesis. The segmental nephron identity of B1R immunoreactivity was determined by co-staining with markers of the collecting duct (Dolichos biflorus), proximal tubule (Dolichos tetraglonus), and nephron progenitors (Pax2). At E14.5, the B1R was confined to few cells in the metanephric mesenchyme. Abundance of B1R increased progressively during development. On E17.5, B1R was enriched in differentiating proximal tubular cells and by postnatal day 1, B1R was clearly expressed on the luminal aspect of the proximal tubule. Quantitative real-time PCR revealed that the levels of B1R mRNA more than double during renal maturation. We conclude that: 1) B1R expression correlates closely with nephron maturation; 2) lack of B1R in nephron progenitors suggests that B1R is unlikely to play a role in early nephrogenesis; and 3) enrichment of B1R in maturing proximal tubule suggests a potential role for this receptor in terminal differentiation of the proximal nephron. |
| Databáze: | OpenAIRE |
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