Structure and inflammatory activity of the LPS isolated from Acetobacter pasteurianus CIP103108

Autor: Eric Giraud, Katarzyna A. Duda, Antonio Molinaro, Djamel Gully, Fabio A. Facchini, Mateusz Pallach, Flaviana Di Lorenzo, Alba Silipo, Francesco Peri
Přispěvatelé: Department of Chemical Sciences, Complesso Universitario Monte Sant'Angelo, Università degli studi di Napoli Federico II, Department of Biotechnology and Biosciences, University of Milano-Bicocca, Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Leibniz Lung Center, Junior Group of Allergobiochemistry, Research Center Borstel Borstel, European Commission [H2020-MSCA-ETN-642157], University of Naples Federico II = Università degli studi di Napoli Federico II, Università degli Studi di Milano-Bicocca = University of Milano-Bicocca (UNIMIB), Research Center Borstel - Leibniz Lung Center [Germany], European Project: 642157,H2020,H2020-MSCA-ITN-2014,TOLLerant(2015), Pallach, M., DI LORENZO, Flaviana, Facchini, F. A., Gully, D., Giraud, E., Peri, F., Duda, K. A., Molinaro, A., Silipo, A., Pallach, M, Di Lorenzo, F, Facchini, F, Gully, D, Giraud, E, Peri, F, Duda, K, Molinaro, A, Silipo, A, Università degli Studi di Milano-Bicocca [Milano] (UNIMIB), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Pallach, Mateusz, Di Lorenzo, Flaviana, Facchini, Fabio Alessandro, Gully, Djamel, Giraud, Eric, Peri, Francesco, Duda, Katarzyna A., Molinaro, Antonio, Silipo, Alba
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Lipopolysaccharides
Magnetic Resonance Spectroscopy
lipopolysaccharides (LPS)
Lipopolysaccharide
Polymers
acétobacter pasteurianus
Biochemistry
Lipid A
chemistry.chemical_compound
activité immunostimulante
Structural Biology
Tandem Mass Spectrometry
gram negative bacteria
CHIM/06 - CHIMICA ORGANICA
Lipopolysaccharides (LPS)
innate immunity
Innate immunity
biology
Chemistry
Kefir
Fatty Acids
Monosaccharides
General Medicine
Polymères
MD2-TLR4
Gram-negative bacteria
kéfir
Cell envelope
Inflammation Mediators
Autre (Sciences du Vivant)
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
medicine.drug_class
Acetobacter pasteurianuss
Immunostimulant
Microbiology
03 medical and health sciences
Structure-Activity Relationship
medicine
Acetobacter
Humans
Molecular Biology
Innate immune system
lps
biology.organism_classification
Toll-Like Receptor 4
030104 developmental biology
[CHIM.POLY]Chemical Sciences/Polymers
CHIM/08 - CHIMICA FARMACEUTICA
bactérie gram négatif
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Acetobacter pasteurianu
Bacteria
Zdroj: International Journal of Biological Macromolecules
International Journal of Biological Macromolecules, Elsevier, 2018, 119, pp.1027-1035. ⟨10.1016/j.ijbiomac.2018.08.035⟩
International Journal of Biological Macromolecules, 2018, 119, pp.1027-1035. ⟨10.1016/j.ijbiomac.2018.08.035⟩
International Journal of Biological Macromolecules (119), 1027-1035. (2018)
ISSN: 0141-8130
DOI: 10.1016/j.ijbiomac.2018.08.035⟩
Popis: Acetobacter pasteurianus is an acetic acid-producing Gram-negative bacterium commonly found associated with plants and plant products and widely used in the production of fermented foods, such as kefir and vinegar. Due to the acid conditions of the bacterium living habitat, uncommon structural features composing its cell envelope are expected. In the present work we have investigated the A. pasteurianus CIP103108 lipopolysaccharide (LPS) structure and immunoactivity. The structure of the lipid A and of two different O-polysaccharides was assessed. Furthermore, immunological studies with human cells showed a low immunostimulant activity of the isolated LPS, in addition to a slight capability to lower the NF-kB activation upon stimulation by toxic LPS.
Databáze: OpenAIRE
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