Effects of iloprost, a stable prostacyclin analog, on intestinal leukocyte adherence and microvascular blood flow in rat experimental endotoxemia
| Autor: | Jan Dettmann, Christian Lehmann, Wolfgang J. Kox, Jens-Peter König, Jürgen Birnbaum |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Lipopolysaccharide medicine.medical_treatment Prostacyclin Critical Care and Intensive Care Medicine chemistry.chemical_compound In vivo Internal medicine Intestine Small Cell Adhesion Laser-Doppler Flowmetry Leukocytes Medicine Animals Iloprost Prospective Studies Rats Wistar Chemotherapy Analysis of Variance Microscopy Video business.industry Microcirculation Hemodynamics Blood flow Laser Doppler velocimetry Small intestine Endotoxemia Rats Endocrinology medicine.anatomical_structure chemistry Microscopy Fluorescence business Blood Flow Velocity Platelet Aggregation Inhibitors medicine.drug |
| Zdroj: | Critical care medicine. 29(7) |
| ISSN: | 0090-3493 |
| Popis: | Objective: To investigate the effects of iloprost, a stable prostacyclin analog, on leukocyte adherence in intestinal venules and intestinal microvascular blood flow in experimental endotoxemia. Design: Prospective, randomized, controlled animal study. Setting: Experimental laboratory. Subjects: Twenty-one male Wistar rats weighing 190 ± 40 g. Interventions: The rats were divided equally into three groups: the first was a control group; the second received endotoxin (20 mg/kg lipopolysaccharide from Escherichia coli 055:B5 intravenously); and the third received endotoxin and intravenous iloprost infusion (2 ng.kg -1 .min -1 ). Measurements and Main Results: The distal small intestine of the animals was examined by using intravital fluorescence videomicroscopy 2 hrs after endotoxin challenge. Leukocytes were stained in vivo by means of rhodamine 6G. Intestinal microvascular blood flow was measured by laser Doppler flowmetry in the terminal ileum. Iloprost treatment significantly attenuated the count of adherent leukocytes in collecting venules (control, 61 ± 10 n/mm 2 ; lipopolysaccharide, 364 ± 60 n/mm 2 ; iloprost, 232 ± 29 n/mm 2 ; p |
| Databáze: | OpenAIRE |
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