Toxicological effects of acrylamide on rat testicular gene expression profile
| Autor: | Chang-Hoon Han, Jin-Hyang Choi, Dong-Un Han, Yong Jin, Hye-Jin Yang, Sang-Hyun Lee, Chanhee Chae, Mun-Han Lee |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Programmed cell death Cell Survival Administration Oral Down-Regulation Gene Expression Apoptosis Biology Testicle Toxicology Andrology Rats Sprague-Dawley Internal medicine Testis medicine Animals Oligonucleotide Array Sequence Analysis Acrylamide Leydig cell Dose-Response Relationship Drug Sperm Count urogenital system Cell growth Gene Expression Profiling Leydig Cells Epididymis Sperm Spermatozoa Rats Up-Regulation Endocrinology medicine.anatomical_structure Environmental Pollutants Spermatogenesis |
| Zdroj: | Reproductive toxicology (Elmsford, N.Y.). 19(4) |
| ISSN: | 0890-6238 |
| Popis: | Toxicological effects of acrylamide on differential gene expression profile of rat testis were evaluated. Acrylamide induced morphological sperm defects, and decreased sperm concentration in cauda epididymis. Serum testosterone level and Leydig cell viability were also decreased dose-dependently, which resulted in decreased spermatogenesis. Acrylamide-induced histopathological lesions, such as formation of multinucleated giant cells and vacuolation, and numerous apoptotic cells were observed in seminiferous tubules. cDNA microarray analysis revealed that genes related to testicular-functions, apoptosis, cellular redox, cell growth, cell cycle, and nucleic acid-binding were up/down-regulated in testes isolated from acrylamide-treated group (60 mg/kg/day). Acrylamide toxicity appears to increase Leydig cell death and perturb gene expression levels, contributing to sperm defects and various abnormal histopathological lesions including apoptosis in rat testis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect