The effects of blood on rapid urease testing for Helicobacter pylori in mucosal biopsies from the gastric antrum
| Autor: | M. M. Soong, C. W. Koh, T. Y. Kong, John Lee, Colm O'Morain, Niall Breslin, M. Gopaul |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
medicine.medical_specialty Urease Peptic Rapid urease test Gastroenterology Sensitivity and Specificity Helicobacter Infections Predictive Value of Tests Internal medicine medicine Pyloric Antrum Humans In patient Gastric antrum Aged biology medicine.diagnostic_test Helicobacter pylori business.industry Histology General Medicine Gold standard (test) Middle Aged digestive system diseases Endoscopy Blood Peptic Ulcer Hemorrhage Gastric Mucosa biology.protein Reagent Kits Diagnostic business |
| Zdroj: | Irish journal of medical science. 169(1) |
| ISSN: | 0021-1265 |
| Popis: | While the eradication ofHelicobacter pylori in patients with bleeding peptic ulcer disease (PUD) decreases the rate of ulcer re-bleeding, the sensitivity of the rapid urease test (RUT) forH. pylori diagnosis is lower in this setting. The aim of this study was therefore to determine if exposing a gastric biopsy specimen to blood before its use in the RUT (CLOtest) could account for these findings. In patients undergoing endoscopy for the evaluation of dyspepsia gastric mucosal biopsies were obtained forH. pylori diagnosis (RUT, microbiology, and histology). Mucosal biopsies from each patient were also exposed to blood for 15, 30 and 45 minutes before use in the RUT. Using a combination of diagnostic tests (histology, microbiology and routine CLOtest) as the ‘gold standard’, the sensitivity, specificity, positive predictive value and negative predictive value of the CLOtest remained above 90% despite prior exposure of the gastric biopsy specimen to blood, and these values were not significantly different from the performance characteristics of the CLOtest processed in a routine manner. The exposure of gastric mucosal biopsy specimens to blood alone is not the explanation for the reduced sensitivity of the RUT in patients with bleeding peptic ulcers. |
| Databáze: | OpenAIRE |
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