Honokiol ameliorates radiation-induced brain injury via the activation of SIRT3
| Autor: | Hongli Yang, Zhihong Zhao, Guixiang Liao, Xiaming Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Honokiol ionizing irradiation Medicine (General) SIRT3 Radiation induced sirtuin 3 medicine.disease_cause Biochemistry Lignans Pre-Clinical Research Report 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine R5-920 Medicine Animals Zebrafish radiation-induced brain injury chemistry.chemical_classification reactive oxygen species Reactive oxygen species biology business.industry Biochemistry (medical) Biphenyl Compounds Brain Cell Biology General Medicine Ionizing irradiation biology.organism_classification Cell biology Oxidative Stress 030104 developmental biology chemistry cyclooxygenase-2 030220 oncology & carcinogenesis Brain Injuries business Oxidative stress |
| Zdroj: | Journal of International Medical Research, Vol 48 (2020) The Journal of International Medical Research |
| ISSN: | 1473-2300 |
| Popis: | Objective Sirtuin 3 (SIRT3) plays a vital role in regulating oxidative stress in tissue injury. The aim of this study was to evaluate the radioprotective effects of honokiol (HKL) in a zebrafish model of radiation-induced brain injury and in HT22 cells. Methods The levels of reactive oxygen species (ROS), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) were evaluated in the zebrafish brain and HT22 cells. The expression levels of SIRT3 and cyclooxygenase-2 (COX-2) were measured using western blot assays and real-time polymerase chain reaction (RT-PCR). Results HKL treatment attenuated the levels of ROS, TNF-α, and IL-1β in both the in vivo and in vitro models of irradiation injury. Furthermore, HKL treatment increased the expression of SIRT3 and decreased the expression of COX-2. The radioprotective effects of HKL were achieved via SIRT3 activation. Conclusions HKL attenuated oxidative stress and pro-inflammatory responses in a SIRT3-dependent manner in radiation-induced brain injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|