Labd-14-ene-8,13-diol (sclareol) induces cell cycle arrest and apoptosis in human breast cancer cells and enhances the activity of anticancer drugs
| Autor: | Sophia Hatziantoniou, P. Pantazis, Magdalini Papadaki, Costas Demetzos, Konstantinos Alevizopoulos, Kostas Dimas, C. Tsimplouli |
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| Rok vydání: | 2006 |
| Předmět: |
Programmed cell death
Time Factors Cell cycle checkpoint Antineoplastic Agents Apoptosis Breast Neoplasms Biology Resting Phase Cell Cycle S Phase chemistry.chemical_compound Cell Line Tumor Humans Cytotoxic T cell Drug Interactions Cell Proliferation Etoposide Pharmacology Dose-Response Relationship Drug Sclareol Cell Cycle G1 Phase General Medicine Cell cycle chemistry Doxorubicin Cell culture Cancer cell Cancer research Female Cisplatin Diterpenes Tumor Suppressor Protein p53 |
| Zdroj: | Biomedicine & Pharmacotherapy. 60:127-133 |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2006.01.003 |
| Popis: | Sclareol is a labdane-type diterpene that has demonstrated a significant cytotoxic activity against human leukemic cell lines. Here, we report the effect of sclareol against the human breast cancer cell lines MN1 and MDD2 derived from the parental cell line, MCF7. MN1 cells express functional p53, whereas MDD2 cells do not express p53. Flow cytometry analysis of the cell cycle indicated that sclareol was able to inhibit DNA synthesis induce arrest at the G(0/1) phase of the cycle apoptosis independent of p53. Sclareol-induced apoptosis was further assessed by detection of fragmented DNA in the cells. Furthermore, sclareol enhanced the activity of known anticancer drugs, doxorubicin, etoposide and cisplatinum, against MDD2 breast cancer cell line. |
| Databáze: | OpenAIRE |
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