Spatiotemporal pattern of calmodulin and [Ca2+]iis related to resumption of meiosis in mouse oocytes
| Autor: | Chao-Jun Li, Gu Dai, Chun-Ming Bi, Xi-Ran Zhang, Yao-Chun Wu, Yan Chen |
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| Rok vydání: | 2004 |
| Předmět: |
IBMX
Calmodulin chemistry.chemical_element Calcium Biology law.invention Mice chemistry.chemical_compound Meiosis Confocal microscopy law medicine Animals Calcium Signaling Receptor Fluorescent Dyes Cell Nucleus Microscopy Confocal Germinal vesicle Cell Biology General Medicine Oocyte Cell biology medicine.anatomical_structure chemistry Oocytes biology.protein Female |
| Zdroj: | Cell Biology International. 28:317-322 |
| ISSN: | 1065-6995 |
| DOI: | 10.1016/j.cellbi.2004.01.011 |
| Popis: | During meiotic maturation, mammalian oocytes undergo a series of morphological and physiological changes that prepare them for fertilization. Calcium-initiated signaling is thought to trigger these processes. In this study, we examine the spatio-temporal pattern of calcium and calmodulin (CaM), its downstream receptor, in order to investigate their association with meiotic maturation. Intracellular free calcium and activated CaM levels were measured using the fluorescent probes Calcium Green-1 and TA-CaM, respectively. The distribution patterns were examined using confocal microscopy. Both calcium and activated CaM showed a dynamic spatiotemporal distribution during meiotic maturation. After release from IBMX buffer, calcium was found to periodically translocate from the perinuclear region to the germinal vesicle (GV) in 90 s intervals. After 90 min, calcium stopped oscillating and became concentrated within the GV. After a further 60 min, the GV broke down and calcium dispersed into the ooplasm, but calcium levels were slightly lower here than in the original nuclear region. Activated CaM also showed a dynamic patterning process similar to calcium. Taking the data from calcium chelation and CaM inhibition together, our results suggest that the dynamic distribution patterns of calcium and activated CaM are crucial for oocyte maturation. |
| Databáze: | OpenAIRE |
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