Generation and protective efficacy of a cold-adapted attenuated genotype 2b porcine epidemic diarrhea virus
| Autor: | Hokeun Won, Dong-Uk Lee, Changhee Lee, Seung-Chul Lee, Hwan Won Choi, In Joong Yoon, Yun Hee Noh, Guehwan Jang, Han Sang Yoo |
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| Rok vydání: | 2019 |
| Předmět: |
Genotype
040301 veterinary sciences Swine Sus scrofa Vaccines Attenuated Microbiology Virus 0403 veterinary science 03 medical and health sciences Random Allocation Attenuated vaccine Immunity Pregnancy Chlorocebus aethiops medicine Animals Vero Cells 030304 developmental biology Swine Diseases 0303 health sciences whole genome sequencing General Veterinary biology Porcine epidemic diarrhea virus Viral Vaccines 04 agricultural and veterinary sciences biology.organism_classification protection Virology Vaccination Cold Temperature Diarrhea Animals Newborn cold adaptation Vero cell Colostrum Female Original Article medicine.symptom Coronavirus Infections |
| Zdroj: | Journal of Veterinary Science |
| ISSN: | 1976-555X |
| Popis: | The recent emergence and re-emergence of porcine epidemic diarrhea virus (PEDV) underscore the urgent need for the development of novel, safe, and effective vaccines against the prevailing strain. In this study, we generated a cold-adapted live attenuated vaccine candidate (Aram-P29-CA) by short-term passage of a virulent PEDV isolate at successively lower temperatures in Vero cells. Whole genome sequencing identified 12 amino acid changes in the cold-adapted strain with no insertions and deletions throughout the genome. Animal inoculation experiments confirmed the attenuated phenotype of Aram-P29-CA virus in the natural host. Pregnant sows were orally administered P29-CA live vaccines two doses at 2-week intervals prior to parturition, and the newborn piglets were challenged with the parental virus. The oral homologous prime-boost vaccination of P29-CA significantly improved the survival rate of the piglets and notably mitigated the severity of diarrhea and PEDV fecal shedding after the challenge. Furthermore, strong antibody responses to PEDV were detected in the sera and colostrum of immunized sows and in the sera of their offspring. These results demonstrated that the cold-adapted attenuated virus can be used as a live vaccine in maternal vaccination strategies to provide durable lactogenic immunity and confer passive protection to litters against PEDV. |
| Databáze: | OpenAIRE |
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