Collagen nanofibres are a biomimetic substrate for the serum-free osteogenic differentiation of human adipose stem cells
| Autor: | Rebekah A. Neal, Roy C. Ogle, Adam J. Katz, Anna M. Parker, Stephanie N. Kaszuba, Lauren S. Sefcik, Edward A. Botchwey |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Cell Survival Cellular differentiation Biomedical Engineering Medicine (miscellaneous) Adipose tissue Anthraquinones Matrix (biology) Culture Media Serum-Free Article Biomaterials Extracellular matrix Biomimetic Materials Osteogenesis Adipocytes Animals Humans Pseudopodia Osteopontin Cell Shape Cells Cultured biology Reverse Transcriptase Polymerase Chain Reaction Chemistry Stem Cells Cell Cycle Cell Differentiation Nanostructures Rats Cell biology Gene Expression Regulation Biochemistry biology.protein Alkaline phosphatase Female Collagen Stem cell Osteonectin |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 2:210-220 |
| ISSN: | 1932-7005 1932-6254 |
| DOI: | 10.1002/term.85 |
| Popis: | Electrospinning has recently gained widespread attention as a process capable of producing nanoscale fibres that mimic native extracellular matrix. In this study, we compared the osteogenic differentiation behaviour of human adipose stem cells (ASCs) on a 3D nanofibre matrix of type I rat tail collagen (RTC) and a 2D RTC collagen-coated substrate, using a novel serum-free osteogenic medium. The serum-free medium significantly enhanced the numbers of proliferating cells in culture, compared to ASCs in traditional basal medium containing 10% animal serum, highlighting a potential clinical role for in vitro stem cell expansion. Osteogenic differentiation behaviour was assessed at days 7, 14 and 21 using quantitative real-time RT-PCR analysis of the osteogenic genes collagen I (Coll I), alkaline phosphatase (ALP), osteopontin (OP), osteonectin (ON), osteocalcin (OC) and core-binding factor-alpha (cbfa1). All genes were upregulated (>one-fold) in ASCs cultured on nanofibre scaffolds over 2D collagen coatings by day 21. Synthesis of mineralized extracellular matrix on the scaffolds was assessed on day 21 with Alizarin red staining. These studies demonstrate that 3D nanoscale morphology plays a critical role in regulating cell fate processes and in vitro osteogenic differentiation of ASCs under serum-free conditions. |
| Databáze: | OpenAIRE |
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