Protective effect of seleno-L-methionine on cyclophosphamide-induced urinary bladder toxicity in rats
| Autor: | Gokhan Bayramoglu, Ruhi Uyar, Hakan Senturk, Suzan Yaman, Adnan Ayhanci, Sibel Gunes, Yılmaz Altuner, Varol Sahinturk, Sila Appak |
|---|---|
| Přispěvatelé: | Appak, Sıla, Izmir Institute of Technology. Molecular Biology and Genetics |
| Rok vydání: | 2009 |
| Předmět: |
Male
Cyclophosphamide Endocrinology Diabetes and Metabolism Clinical Biochemistry Urinary Bladder Pharmacology Biochemistry Cytoprotectivity Inorganic Chemistry Lipid peroxidation chemistry.chemical_compound Random Allocation Edema medicine Animals Humans Urothelium Selenomethionine Urotoxicity Antineoplastic Agents Alkylating Urinary bladder Chemistry Rattus Biochemistry (medical) General Medicine medicine.disease Extravasation Seleno-l-methionine Rats medicine.anatomical_structure Toxicity Immunology Female medicine.symptom Reactive Oxygen Species Oxidation-Reduction medicine.drug Hemorrhagic cystitis |
| Zdroj: | Biological trace element research. 134(1) |
| ISSN: | 1559-0720 |
| Popis: | Cyclophosphamide (CP) is a widely used antineoplastic drug, which could cause toxicity of the normal cells due to its toxic metabolites. Its urotoxicity may cause dose-limiting side effects like hemorrhagic cystitis. Overproduction of reactive oxygen species (ROS) during inflammation is one of the reasons of the urothelial injury. Selenoproteins play crucial roles in regulating ROS and redox status in nearly all tissues; therefore, in this study, the urotoxicity of CP and the possible protective effects of seleno-l-methionine (SLM) on urinary bladder of rats were investigated. Intraperitoneal (i.p.) administration of 50, 100, or 150 mg/kg CP induced cystitis, in a dose-dependent manner, as manifested by marked congestion, edema and extravasation in rat urinary bladder, a marked desquamative damage to the urothelium, severe inflammation in the lamina propria, focal erosions, and polymorphonuclear (PMN) leukocytes associated with occasional lymphocyte infiltration determined by macroscopic and histopathological examination. In rat urinary bladder tissue, a significant decrease in the endogenous antioxidant compound glutathione, and elevation of lipid peroxidation were also noted. Pretreatment with SLM (0.5 or 1 mg/kg) produced a significant decrease in the bladder edema and caused a marked decrease in vascular congestion and hemorrhage and a profound improvement in the histological structure. Moreover, SLM pretreatment decreased lipid peroxide significantly in urinary bladder tissue, and glutathione content was greatly restored. These results suggest that SLM offers protective effects against CP-induced urinary bladder toxicity and could be used as a protective agent against the drug toxicity. © 2009 Humana Press Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink