Evaluation of the Effects of Nobiletin on Toll-Like Receptor 3 Signaling Pathways in Prostate Cancer In Vitro
| Autor: | Asuman Deveci Ozkan, Süleyman Kaleli, Mehmet Sarihan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Cancer Research Flavonoid Medicine (miscellaneous) Nobiletin 03 medical and health sciences chemistry.chemical_compound Prostate cancer 0302 clinical medicine Humans Medicine Receptor chemistry.chemical_classification Toll-like receptor 030109 nutrition & dietetics Nutrition and Dietetics business.industry Prostatic Neoplasms food and beverages Cancer Flavones medicine.disease Toll-Like Receptor 3 Oncology chemistry 030220 oncology & carcinogenesis TLR3 Cancer research Signal transduction business Signal Transduction |
| Zdroj: | Nutrition and Cancer. 73:1138-1144 |
| ISSN: | 1532-7914 0163-5581 |
| Popis: | Nobiletin as a nontoxic dietary citrus flavonoid has anticancer effects in cancer. Toll-like receptor three has a role in prostate cancer progression. However the relationship among NOB and TLR3 signaling in PCa has not been elucidated, yet. Therefore, we aimed to evaluate the effects of NOB on the activation of TLR3 signaling pathways in PCa In Vitro. PC-3, LNCaP and HUVEC cells were used for comparison of NOB-mediated TLR3 signaling pathways. After treatment with NOB and Poly I:C alone and NOB + Poly I:C, RT-PCR, western blotting and ELISA assay were performed to evaluate changes in gene and protein expression level, as well as CASP8. NOB potentially induced TLR3/IRF3 signaling pathway and the activation of TLR3/IRF3 signaling pathway by both NOB and Poly I:C was more profound in LNCaP than PC-3 cells. However, the level of TRIF protein and CASP8 decreased after both NOB and Poly I:C incubation. NOB could mediate TLR3 signaling pathways. NOB + Poly I:C could improve the activation of TLR3/IRF3 signaling pathway. However, the activation of TRIF/RIPK1/FADD signaling pathway reduced. Therefore, the elucidation of molecular mechanisms of TLR3 signaling pathways and the combination effects of NOB + Poly I:C on apoptotic cell death are further studied. |
| Databáze: | OpenAIRE |
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