Interaction of endothelial cells with a laminin A chain peptide (SIKVAV) in vitro and induction of angiogenic behavior in vivo
| Autor: | Derrick S. Grant, Yoshihiko Yamada, Hynda K. Kleinman, Barbara A. Piasecki, Rafael Fridman, James L. Kinsella, Robert Auerbach, Mona Zain |
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| Rok vydání: | 1992 |
| Předmět: |
Physiology
Angiogenesis Clinical Biochemistry Molecular Sequence Data Extraembryonic Membranes Chick Embryo Cell–cell interaction Laminin Cell Movement medicine Animals Humans Amino Acid Sequence Cells Cultured Yolk Sac Basement membrane Tube formation Matrigel biology Neovascularization Pathologic Cell Biology Peptide Fragments Cell biology Culture Media Endothelial stem cell Chorioallantoic membrane Drug Combinations medicine.anatomical_structure Biochemistry biology.protein Microscopy Electron Scanning Proteoglycans Collagen Endothelium Vascular |
| Zdroj: | Journal of cellular physiology. 153(3) |
| ISSN: | 0021-9541 |
| Popis: | Endothelial cells are known to bind to laminin, and two peptides derived from the laminin A (CTFALRGDNP) and B1(CDPGYIGSR) chains block the capillary-like tube formation on a laminin-rich basement membrane matrix, Matrigel. In the present study, we have used various in vitro and in vivo assays to investigate the angiogenic-biologic effects of a third active site in the laminin A chain, CSRARKQAASIKVAVSADR (designated PA22-2) on endothelial cells. The SIKVAV-containing peptide was as active as the YIGSR-containing peptide for endothelial cell attachment but was less active than either the RGD-containing peptide or intact laminin. Endothelial cells seeded on this peptide appeared fibroblastic with many extended processes, unlike the normal cobblestone morphology observed on tissue culture plastic. In addition, in contrast to normal tube formation on Matrigel, short irregular structures formed, some of which penetrated the matrix and sprouting was more apparent. Analysis of endothelial cell conditioned media of cells cultured in the presence of this peptide indicated degradation of the Matrigel and zymograms demonstrated active collagenase IV (gelatinase) at 68 and 62 Kd. A murine in vivo angiogenesis assay and the chick yolk sac/chorioallantoic membrane assays with the peptide demonstrated increased endothelial cell mobilization, capillary branching, and vessel formation. These data suggest that the -SIKVAV-site may play an important role in initiating branching and formation of new capillaries from the parent vessels, a behavior that is observed in vivo in response to tumor growth or in the normal vascular response to injury. © 1992 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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