Clostridium difficile -derived membrane vesicles induce the expression of pro-inflammatory cytokine genes and cytotoxicity in colonic epithelial cells in vitro
| Autor: | Hyo Il Kwon, Asiimwe Nicholas, Gati Noble Selasi, Chi Won Choi, Yoo Jeong Kim, Seok Hyeon Na, Seung Il Kim, Je Chul Lee, Hyejin Jeon |
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| Rok vydání: | 2017 |
| Předmět: |
Proteomics
0301 basic medicine Colon Bacterial Toxins Interleukin-1beta 030106 microbiology Cell Culture Techniques Biology Microbiology Proinflammatory cytokine Enterotoxins 03 medical and health sciences Bacterial Proteins Microscopy Electron Transmission Intestinal mucosa medicine Humans Secretion Interleukin 8 Intestinal Mucosa Cytotoxicity Chemokine CCL2 Enterocolitis Pseudomembranous Clostridioides difficile Interleukin-6 Monocyte Interleukin-8 Epithelial Cells Hep G2 Cells Flow Cytometry 030104 developmental biology Infectious Diseases medicine.anatomical_structure Gene Expression Regulation Caco-2 Cell culture Cytokines Caco-2 Cells |
| Zdroj: | Microbial Pathogenesis. 107:6-11 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2017.03.006 |
| Popis: | Clostridium difficile is the most common etiological agent of antibiotic-associated diarrhea in hospitalized and non-hospitalized patients. This study investigated the secretion of membrane vesicles (MVs) from C. difficile and determined the expression of pro-inflammatory cytokine genes and cytotoxicity of C. difficile MVs in epithelial cells in vitro. C. difficile ATCC 43255 and two clinical isolates secreted spherical MVs during in vitro culture. Proteomic analysis revealed that MVs of C. difficile ATCC 43255 contained a total of 262 proteins. Translation-associated proteins were the most commonly identified in C. difficile MVs, whereas TcdA and TcdB toxins were not detected. C. difficile ATCC 43255-derived MVs stimulated the expression of pro-inflammatory cytokine genes, including interleukin (IL)-1β, IL-6, IL-8, and monocyte chemoattractant protein-1 in human colorectal epithelial Caco-2 cells. Moreover, these extracellular vesicles induced cytotoxicity in Caco-2 cells. In conclusion, C. difficile MVs are important nanocomplexes that elicit a pro-inflammatory response and induce cytotoxicity in colonic epithelial cells, which may contribute, along with toxins, to intestinal mucosal injury during C. difficile infection. |
| Databáze: | OpenAIRE |
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