Intraperitoneal administration of CDP-choline or a combination of cytidine plus choline improves nerve regeneration and functional recovery in a rat model of sciatic nerve injury

Autor: Ismail H. Ulus, Necdet Karli, M. Ilker Kafa, Ahmet Bekar, Mehmet Cansev, Basak Caner, M. Ayberk Kurt
Přispěvatelé: Uludağ Üniversitesi/Tıp Fakültesi/Anatomi Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Nöroloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Beyin ve Sinir Cerrahisi Anabilim Dalı., Kafa, Mustafa İlker, Bekar, Ahmet, Kurt, Mustafa Ayberk, Karlı, Necdet, Cansev, Mehmet, AAR-4341-2020, M-9071-2019, AAG-7125-2021
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Experimental focal ischemia
Rats
sprague-dawley
medicine.medical_treatment
Muscle action potential
Electromyography
Cytidine
Disease models
animal
Animal tissue
Choline
chemistry.chemical_compound
Metabolites
Axon
Saline
medicine.diagnostic_test
Brain
Axotomy
General Medicine
Sciatic nerve injury
Nerve fiber
Injections
intraperitoneal
medicine.anatomical_structure
Neurology
Anesthesia
Clinical neurology
Peripheral nerve injury
lipids (amino acids
peptides
and proteins)
Female
Sciatic nerve
medicine.drug
Citicoline
Neuroprotective Agents
Glycerylphosphorylcholine
Drug therapy
combination
Neurosciences & neurology
Article
medicine
Animals
Nootropic agents
Animal model
Animal experiment
Uridine
Cytidine diphosphate choline
business.industry
Autotomy
Recovery of function
Neurosciences
Sciatic neuropathy
medicine.disease
Phospholipase a(2)
Nonhuman
Rats
carbohydrates (lipids)
Nerve regeneration
Drug effect
chemistry
Phosphocholine
Stimulation
Rat
Neurology (clinical)
business
Transient cerebral-ischemia
Embolic stroke
Controlled study
Popis: Objective: Topical cytidine-5'-diphosphocholine (CDP-choline) improves functional recovery and promotes nerve regeneration in sciatic nerve injury in rats. The aims of this study were to test whether systemic treatment with CDP-choline was effective in improving the recovery of injured sciatic nerve, and to determine whether the cytidine and/or choline moieties of CDP-choline contribute to its beneficial actions. Methods: Seventy Sprague-Dawley rats underwent a surgical procedure that involved transectioning and immediate surgical repairing of the right sciatic nerve. Rats were assigned to one of five groups and administered intraperitoneally 1 ml/kg of saline ( control) or saline containing 600 mmol/kg of each of CDPcholine, cytidine, choline, or cytidine + choline. Results: Recovery in sciatic function index score was greater in rats treated with CDP-choline, choline, or cytidine + choline at 8 and 12 weeks after the interventions. Peripheral nerve regeneration evaluated by electromyography at 12 weeks was also greater in rats receiving CDP-choline ( 228% of control), choline ( 168% of control), or cytidine + choline ( 221% of control). Axon counts and axon density increased significantly following CDP-choline, choline, or cytidine + choline, respectively. Treatment with equivalent dose of cytidine failed to affect sciatic function index, electromyography, and axon counts. Treatment with CDP-choline, but not its metabolites improved nerve adherence and separability score. Conclusion: These data show that intraperitoneal CDP-choline, as well as the combination of its metabolites, cytidine + choline, improves functional recovery and promotes regeneration of injured sciatic nerves in rats. CDP-choline also improves nerve adherence and separability.
Databáze: OpenAIRE
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