Validation of a high-performance liquid chromatographic method for the enantiospecific quantitation of zopiclone in plasma
| Autor: | Christopher P Alderman, Markus G Gebauer |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Calibration curve
Coefficient of variation Metabolite Clinical Biochemistry Biochemistry Piperazines Analytical Chemistry chemistry.chemical_compound Drug Discovery medicine Hypnotics and Sedatives Molecular Biology Chromatography High Pressure Liquid Pharmacology Detection limit Zopiclone Chromatography Chemistry Stereoisomerism General Medicine Reference Standards Desmethyl Racemic mixture Enantiomer Azabicyclo Compounds medicine.drug |
| Zdroj: | Biomedical Chromatography. 16:241-246 |
| ISSN: | 1099-0801 0269-3879 |
| Popis: | Zopiclone is a hypnosedative with clinical effects similar to benzodiazepines but thought to have less potential for rebound insomnia and withdrawal effects. Zopiclone is administered as a racemic mixture, and an enantiospecific method of analysis of zopiclone in plasma is desirable in the study of pharmacokinetic drug interactions. We report a modification of an HPLC method reported by Foster et al. using a closely related structural analogue of zopiclone as internal standard. Zopiclone was detected at 306 nm and linear calibration curves were constructed in the range of 1.0–250 ng/mL for each enantiomer. The % CV at 2.5 ng/mL was 12.0% for (−)-zopiclone and 14.3% for (+)-zopiclone, and the limit of quantification of each enantiomer was 2.5 ng/mL. At higher concentrations, the coefficient of variation was less than 10%. The nominal concentration of quality control samples was predicted with an accuracy within a range of ±11.6%. The method was used in the analysis of plasma obtained from psychiatric patients. One sample obtained following a non-fatal overdose with zopiclone contained the metabolites (−)-N-oxide zopiclone and both enantiomers of desmethyl zopiclone. The metabolite enantiomers were resolved on the column with retention times similar to zopiclone. The N-oxide metabolite co-eluted with internal standard. Copyright © 2002 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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